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Q0BWB5 (SYL_HYPNA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:HNE_3557
OrganismHyphomonas neptunium (strain ATCC 15444) [Complete proteome] [HAMAP]
Taxonomic identifier228405 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesHyphomonadaceaeHyphomonas

Protein attributes

Sequence length855 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 855855Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334764

Regions

Motif45 – 5511"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0BWB5 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 40A93E3F4848CAFA

FASTA85594,333
        10         20         30         40         50         60 
MATRYDPQSA EPRWRDAWEK ADIFRTKAPK DAPGAPKAFV LEMFPYPSGR LHMGHVRNYA 

        70         80         90        100        110        120 
MGDVVARHKR AKGYNVLHPM GWDAFGMPAE NAAMERKVHP GKWTYANIES MKAQFRKLGL 

       130        140        150        160        170        180 
SLDWSREFAT CDPDYYGAQQ ALFLKLMDKG LVYRKASKVN WDPVDNTVLA NEQVIDGRGW 

       190        200        210        220        230        240 
RSGAPVEQRE LTQWFFKITA YADDLLEAVQ KLERWPEKVR TMQANWIGRS EGLEMTFAFD 

       250        260        270        280        290        300 
GERPAGFEDG ISVFTTRPDT LFGASFVALS PDHPLTLQLA EKSDALQAFR AKCAQIGTSE 

       310        320        330        340        350        360 
EAIEKAEKLG FDTGLTVAHP FEPGRTVPVW VANFVLMGYG TGAIFGCPAH DQRDLDFARK 

       370        380        390        400        410        420 
FGLDVFPVVL PPGADAAAFE VGIEAYTGPG HIYKSGFLDG LSIDDAKRAA IAKIEAAGQG 

       430        440        450        460        470        480 
EGKVNYRLRD WGVSRQRYWG CPIPVVHCED CGVVGVPAAD LPVRLPEDVT FDVPGNPLDR 

       490        500        510        520        530        540 
HPDWKHVDCP KCGKPARRET DTLDTFVDSS WYYARFASVS DPEERAYWLP VDQYIGGVEH 

       550        560        570        580        590        600 
AVLHLLYSRF FSRAMRDVGE LDLPSGEPFA GLFTQGMVTH ETYRSEGGTW LEPSAVERKD 

       610        620        630        640        650        660 
GQVFEIATGK PVKVGAIEKM SKSKKNTVDP DAIVATYGAD VARWFVLSDS PPERDVEWTQ 

       670        680        690        700        710        720 
SGAEGAARFV QRVWSFVDSL PETGPFPAPG SDDVSTALRK SNHKAVAAID RAIEEFRFNS 

       730        740        750        760        770        780 
AIATIHEWVN ALKKTESDPA TLGARAEGAD MLARCLVPFM PHLAEACWER LGQTSLVSQA 

       790        800        810        820        830        840 
MWPKIDASLV VDDTVTLAVQ VNGKRRAEIT VAKDMAPDAV EAAAKALPDV ASFIAGKSVK 

       850 
KTIVVPGRIV NIVVA 

« Hide

References

[1]"Comparative genomic evidence for a close relationship between the dimorphic prosthecate bacteria Hyphomonas neptunium and Caulobacter crescentus."
Badger J.H., Hoover T.R., Brun Y.V., Weiner R.M., Laub M.T., Alexandre G., Mrazek J., Ren Q., Paulsen I.T., Nelson K.E., Khouri H.M., Radune D., Sosa J., Dodson R.J., Sullivan S.A., Rosovitz M.J., Madupu R., Brinkac L.M. expand/collapse author list , Durkin A.S., Daugherty S.C., Kothari S.P., Giglio M.G., Zhou L., Haft D.H., Selengut J.D., Davidsen T.M., Yang Q., Zafar N., Ward N.L.
J. Bacteriol. 188:6841-6850(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15444.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000158 Genomic DNA. Translation: ABI77585.1.
RefSeqYP_762228.1. NC_008358.1.

3D structure databases

ProteinModelPortalQ0BWB5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING228405.HNE_3557.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI77585; ABI77585; HNE_3557.
GeneID4289011.
KEGGhne:HNE_3557.
PATRIC32220001. VBIHypNep17450_3563.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAPVIHCPK.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycHNEP228405:GI69-3558-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_HYPNA
AccessionPrimary (citable) accession number: Q0BWB5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 17, 2006
Last modified: May 14, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries