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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei634 – 6341ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciGBET391165:GHON-5-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:GbCGDNIH1_0005
OrganismiGranulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)
Taxonomic identifieri391165 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGranulibacter
ProteomesiUP000001963 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 875875Leucine--tRNA ligasePRO_0000334761Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi391165.GbCGDNIH1_0005.

Structurei

3D structure databases

ProteinModelPortaliQ0BW99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi631 – 6355"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0BW99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDSITDLQS DQRPDRSYDF ASAERRWQLA WTERACFTVP DVPDPGARTY
60 70 80 90 100
YVLEMFPYPS GQIHMGHVRN YTLGDVVARY KRAQGYQVLH PMGWDAFGLP
110 120 130 140 150
AENAARERGV HPGQWTWNNI AAMRGELQRM GLSITWEREF ATCDPSYYGH
160 170 180 190 200
QQALFLDFLK KNLVERRESW VNWDPVDETV LANEQVIDGK GWRSGAPVER
210 220 230 240 250
KKLSQWFLRI TDYAEELLAG LDQLDRWPER VRVMQSRWIG RSEGARLRFP
260 270 280 290 300
LVEPLGDQRE IEVYTTRPDT LYGMSFVAIA ADHPVAAALA AHHPALAAFV
310 320 330 340 350
AECRSLGTSE AAIEAAEKRG FDTGLRVKHP FCDETFPVWI ANFVLMDYGT
360 370 380 390 400
GAVFGCPAHD QRDLDFARKY DLSVTPVVLP SDQDAASFTI GRKAYDGDGI
410 420 430 440 450
LFNSGPFDGL TPDAARREAI TRLEAMGWGQ GVTNWRLRDW GVSRQRYWGC
460 470 480 490 500
PIPIIHCDQC GPVPVPADQL PVTLPEDVTF DRPGNPLDHH PSWKHVTCPS
510 520 530 540 550
CGAAAVRETD TFDTFVDSSW YFARFASPHA HVPVLKEAAQ NWLPVDQYIG
560 570 580 590 600
GIEHAILHLL YARFFTRAMA DTGHVPVREP FAGLFTQGMV THESYRAADG
610 620 630 640 650
RWLSPVEVTR HGETVVETAT GEPVQVGRGE KMSKSKRNTV APGEIFNRYG
660 670 680 690 700
ADAARWFILS DNPPERDMEW TDAGAVGAYR FVQRLYRLAE AVARIAKEET
710 720 730 740 750
NRDDASSDAA MTLRRMTHRT VAAVTEALEG FNFNVAVARV YEFANALTEA
760 770 780 790 800
EKKAAEPGMT AARVEAITLL SRIIAPMMPH LAEEMATLIE QGPKLVAEQV
810 820 830 840 850
WPSADPALLV VQSVTIAIQV MGKLRATLDI SPDADQDSVI AQAEADPNVV
860 870
RALEGKRVVK RIYVPNRIVN FVIAG
Length:875
Mass (Da):97,795
Last modified:October 17, 2006 - v1
Checksum:i91357A96A7461C7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000394 Genomic DNA. Translation: ABI60903.1.
RefSeqiWP_011630713.1. NC_008343.1.

Genome annotation databases

EnsemblBacteriaiABI60903; ABI60903; GbCGDNIH1_0005.
KEGGigbe:GbCGDNIH1_0005.
PATRICi22076005. VBIGraBet83793_0005.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000394 Genomic DNA. Translation: ABI60903.1.
RefSeqiWP_011630713.1. NC_008343.1.

3D structure databases

ProteinModelPortaliQ0BW99.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391165.GbCGDNIH1_0005.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI60903; ABI60903; GbCGDNIH1_0005.
KEGGigbe:GbCGDNIH1_0005.
PATRICi22076005. VBIGraBet83793_0005.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciGBET391165:GHON-5-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis."
    Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M.
    J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1260 / CGDNIH1.

Entry informationi

Entry nameiSYL_GRABC
AccessioniPrimary (citable) accession number: Q0BW99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 17, 2006
Last modified: July 22, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.