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Q0BW99 (SYL_GRABC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:GbCGDNIH1_0005
OrganismGranulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) [Complete proteome] [HAMAP]
Taxonomic identifier391165 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGranulibacter

Protein attributes

Sequence length875 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 875875Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334761

Regions

Motif57 – 6711"HIGH" region HAMAP-Rule MF_00049
Motif631 – 6355"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6341ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0BW99 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 91357A96A7461C7D

FASTA87597,795
        10         20         30         40         50         60 
MTDSITDLQS DQRPDRSYDF ASAERRWQLA WTERACFTVP DVPDPGARTY YVLEMFPYPS 

        70         80         90        100        110        120 
GQIHMGHVRN YTLGDVVARY KRAQGYQVLH PMGWDAFGLP AENAARERGV HPGQWTWNNI 

       130        140        150        160        170        180 
AAMRGELQRM GLSITWEREF ATCDPSYYGH QQALFLDFLK KNLVERRESW VNWDPVDETV 

       190        200        210        220        230        240 
LANEQVIDGK GWRSGAPVER KKLSQWFLRI TDYAEELLAG LDQLDRWPER VRVMQSRWIG 

       250        260        270        280        290        300 
RSEGARLRFP LVEPLGDQRE IEVYTTRPDT LYGMSFVAIA ADHPVAAALA AHHPALAAFV 

       310        320        330        340        350        360 
AECRSLGTSE AAIEAAEKRG FDTGLRVKHP FCDETFPVWI ANFVLMDYGT GAVFGCPAHD 

       370        380        390        400        410        420 
QRDLDFARKY DLSVTPVVLP SDQDAASFTI GRKAYDGDGI LFNSGPFDGL TPDAARREAI 

       430        440        450        460        470        480 
TRLEAMGWGQ GVTNWRLRDW GVSRQRYWGC PIPIIHCDQC GPVPVPADQL PVTLPEDVTF 

       490        500        510        520        530        540 
DRPGNPLDHH PSWKHVTCPS CGAAAVRETD TFDTFVDSSW YFARFASPHA HVPVLKEAAQ 

       550        560        570        580        590        600 
NWLPVDQYIG GIEHAILHLL YARFFTRAMA DTGHVPVREP FAGLFTQGMV THESYRAADG 

       610        620        630        640        650        660 
RWLSPVEVTR HGETVVETAT GEPVQVGRGE KMSKSKRNTV APGEIFNRYG ADAARWFILS 

       670        680        690        700        710        720 
DNPPERDMEW TDAGAVGAYR FVQRLYRLAE AVARIAKEET NRDDASSDAA MTLRRMTHRT 

       730        740        750        760        770        780 
VAAVTEALEG FNFNVAVARV YEFANALTEA EKKAAEPGMT AARVEAITLL SRIIAPMMPH 

       790        800        810        820        830        840 
LAEEMATLIE QGPKLVAEQV WPSADPALLV VQSVTIAIQV MGKLRATLDI SPDADQDSVI 

       850        860        870 
AQAEADPNVV RALEGKRVVK RIYVPNRIVN FVIAG 

« Hide

References

[1]"Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis."
Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M.
J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1260 / CGDNIH1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000394 Genomic DNA. Translation: ABI60903.1.
RefSeqYP_743826.1. NC_008343.1.

3D structure databases

ProteinModelPortalQ0BW99.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391165.GbCGDNIH1_0005.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI60903; ABI60903; GbCGDNIH1_0005.
GeneID4274530.
KEGGgbe:GbCGDNIH1_0005.
PATRIC22076005. VBIGraBet83793_0005.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycGBET391165:GHON-5-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_GRABC
AccessionPrimary (citable) accession number: Q0BW99
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 17, 2006
Last modified: May 14, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries