Q0BW57 (FTHS_GRABC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	GbCGDNIH1_0047

Organism

Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) [Complete proteome] [HAMAP]

Taxonomic identifier

391165 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Granulibacter › Granulibacter bethesdensis

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 572	572	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_0000293036
Regions
	Nucleotide binding	81 – 88	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BW57 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 6D804024DA30ADD2	FASTA	572	60,606
	10 20 30 40 50 60 MSTEANKPRG NQHQEAKSDA EIAQAAFMRP IVDVAAEKLG IAAEHLAPYG HYKAKIDLNY 70 80 90 100 110 120 LSSLDSRPDG KLVLVTAISP TPAGEGKTTT TVGLTDALNH IGKKAVACLR EPSLGPCFGV 130 140 150 160 170 180 KGGAAGGGYA QVVPMEDINL HFTGDFHAIG AANNLLAALI DNHVYWGNEL GIDPRRIGWR 190 200 210 220 230 240 RAVDMNDRAL RSIVSSLGGV SNGYPREDGF DITVASEVMA IFCLATDLDD LQRRLGNIIV 250 260 270 280 290 300 GHTKDRKPIR ASELSAAGSM AVLLKDAIAP NLVQTLEHNP AFIHGGPFAN IAHGCNSVIA 310 320 330 340 350 360 TRAALKLSDY VVTEAGFGAD LGAEKFFDIK CRKAGLSPSA VVIVATVRAL KMHGGVAKDA 370 380 390 400 410 420 LKTENVEAVQ KGFANLERHI QNVRKFGVPV VVGVNKFSAD TDAEFQMLHD LCAKMGVPCV 430 440 450 460 470 480 SSDHWANGGA GAADLAHEVV KLVEGGSADF KPLYPEDMPL WDKLRTIATE IYGASDITAD 490 500 510 520 530 540 AAVRKRFDEL QKEGFGHLPI CVAKTQYSFS TDANLRGAPS GHVIPVRDLR LSAGAEFVVA 550 560 570 ICGDIMTMPG LPKVPAANAI RLASNGTIAG LF

References

[1]

"Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis." Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M. J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1260 / CGDNIH1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000394 Genomic DNA. Translation: ABI60945.1.
RefSeq	YP_743868.1. NC_008343.1.
3D structure databases
ProteinModelPortal	Q0BW57.
SMR	Q0BW57. Positions 21-570.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	391165.GbCGDNIH1_0047.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI60945; ABI60945; GbCGDNIH1_0047.
GeneID	4274643.
KEGG	gbe:GbCGDNIH1_0047.
PATRIC	22076091. VBIGraBet83793_0048.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	LKHHGGV.
OrthoDB	EOG6PCPSP.
Enzyme and pathway databases
BioCyc	GBET391165:GHON-47-MONOMER.
UniPathway	UPA00193.
Family and domain databases
Gene3D	3.40.50.300. 2 hits.
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. IPR027417. P-loop_NTPase. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_GRABC

Accession

Primary (citable) accession number: Q0BW57

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	October 17, 2006
Last modified:	May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program