Reviewed, UniProtKB/Swiss-Prot Q0BW40 (SAHH_GRABC)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	GbCGDNIH1_0064

Organism

Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) [Complete proteome] [HAMAP]

Taxonomic identifier

391165 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Granulibacter

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000129284
Regions
	Region	191 – 356	166	NAD binding By similarity
Sites
	Binding site	64	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Binding site	198	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BW40-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 252813E22DAC4FE0	FASTA	440	48,245
	10 20 30 40 50 60 MESSMPDTAV AEDYKVRDIG LADWGRKEIR MAEDEMPGLM ALREEYGNSK PLAGARIAGC 70 80 90 100 110 120 LHMTIQTAVL IETLTALGAT VRWSSCNIFS TQDHAAAGIA AAGIPVFAWK GLSEEEFWWC 130 140 150 160 170 180 IEQTVRGPDG WTPNMILDDG GDLTVLMHDK YPEMLKDVRG LSEETTTGVH RLWEMAKAGK 190 200 210 220 230 240 LLTPAINVND SVTKSKFDNL YGCRESLVDG IRRGTDVMMA GKIAVVAGFG DVGKGSAASL 250 260 270 280 290 300 RNAGCRVLVT EIDPICALQA AMEGYEVVTM DEAASRGDLF VTATGNVDVI TIDHMRAMKN 310 320 330 340 350 360 RAIVCNIGHF DSEIQIESLR NYRWENVKPQ VDEVVFPDGK RLIILSEGRL VNLGNATGHP 370 380 390 400 410 420 SFVMSASFTN QVLAQIELWQ AKPGQYLPNH VYTLPKHLDE KVAALHLAKV GAKLTKLSDK 430 440 QAEYIGVSQA GPFKHDLYRY

References

[1]

"Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis." Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M. J. Bacteriol. 189:8727-8736(2007) [PubMed: 17827295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000394 Genomic DNA. Translation: ABI60962.1.
RefSeq	YP_743885.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BW40.
Genome annotation databases
GeneID	4274660.
GenomeReviews	Gene locus GbCGDNIH1_0064 in contig CP000394_GR.
KEGG	gbe:GbCGDNIH1_0064.
NMPDR	fig|391165.8.peg.67.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0BW40.
OMA	HMRAMKD.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_GRABC

Accession

Primary (citable) accession number: Q0BW40

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)