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Q0BVJ1 (ASSY_GRABC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:GbCGDNIH1_0263
OrganismGranulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) [Complete proteome] [HAMAP]
Taxonomic identifier391165 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGranulibacter

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 416416Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_0000263930

Regions

Nucleotide binding19 – 279ATP By similarity

Sites

Binding site461ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site971Citrulline By similarity
Binding site1021Citrulline By similarity
Binding site1271ATP; via amide nitrogen By similarity
Binding site1291Aspartate By similarity
Binding site1331Aspartate By similarity
Binding site1331Citrulline By similarity
Binding site1341Aspartate By similarity
Binding site1371Citrulline By similarity
Binding site1881Citrulline By similarity
Binding site1971Citrulline By similarity
Binding site2731Citrulline By similarity
Binding site2851Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0BVJ1 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 23AAA2BA61B153C6

FASTA41646,235
        10         20         30         40         50         60 
MTRDIQTMAV KDVKKVVLAY SGGLDTSVIL RWLQTTYNCE VVTFTADLGQ GEELEPARRK 

        70         80         90        100        110        120 
AEMFGVKEIF VDDLRETFVK DFVFPMFRAN AVYEGQYLLG TSIARPLIAQ RQIEIAELTG 

       130        140        150        160        170        180 
ADAVAHGATG KGNDQVRFEL SYYALKPDVK VIAPWREWDL TSRTKLLEFA EANQIPIAKD 

       190        200        210        220        230        240 
KRGEAPFSVD ANLLHSSSEG KLLEDPAEEP DEIVYQRTIS PEAAPDKATI ITIDFEHGDP 

       250        260        270        280        290        300 
VAIDGIRLSP ATLLAKLNEL GKANGIGRLD LVENRFVGMK SRGVYETPGG TILLVAHRGI 

       310        320        330        340        350        360 
ESITLDREAA HLKDSLMPRY AELIYNGFWF SPERRMLQAA IDESQKSVTG RVRLKLYKGN 

       370        380        390        400        410 
TIVIGRESPN SLYSLKHVTF EDDQGAYDQV DAQGFIKLQS LRLRLAAIAG RRGGSL 

« Hide

References

[1]"Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis."
Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M.
J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1260 / CGDNIH1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000394 Genomic DNA. Translation: ABI61161.1.
RefSeqYP_744084.1. NC_008343.1.

3D structure databases

ProteinModelPortalQ0BVJ1.
SMRQ0BVJ1. Positions 15-411.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391165.GbCGDNIH1_0263.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI61161; ABI61161; GbCGDNIH1_0263.
GeneID4274482.
KEGGgbe:GbCGDNIH1_0263.
PATRIC22076553. VBIGraBet83793_0274.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAAPPEEAY.
OrthoDBEOG6K9QCV.

Enzyme and pathway databases

BioCycGBET391165:GHON-268-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_GRABC
AccessionPrimary (citable) accession number: Q0BVJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: October 17, 2006
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways