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Q0BVF4 (THIM_GRABC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyethylthiazole kinase

EC=2.7.1.50
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase
Gene names
Name:thiM
Ordered Locus Names:GbCGDNIH1_0300
OrganismGranulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) [Complete proteome] [HAMAP]
Taxonomic identifier391165 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGranulibacter

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00228

Pathway

Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228

Sequence similarities

Belongs to the Thz kinase family.

Sequence caution

The sequence ABI61198.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 274274Hydroxyethylthiazole kinase HAMAP-Rule MF_00228
PRO_0000383864

Sites

Binding site541Substrate; via amide nitrogen By similarity
Binding site1291ATP By similarity
Binding site1751ATP By similarity
Binding site2021Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0BVF4 [UniParc].

Last modified September 22, 2009. Version 2.
Checksum: 9A0EAD5E832E09AA

FASTA27428,107
        10         20         30         40         50         60 
MTEEPLSAAW LTERTIYLLT LLREKRPLVH NITNLVVTNT TANALLALGA SPAMVEGVEE 

        70         80         90        100        110        120 
VDSFARMADS LVINLGTMSS PRAAAMRLAM AAARQAGKPV VLDPVAVGAL RYRTELARDL 

       130        140        150        160        170        180 
LEAKPHIVRG NASEIIALAG DGGGGRGVDS LVSSTAALEA ASILARRTGG AVAVTGATDY 

       190        200        210        220        230        240 
VTDGTRLIGI ANGHPMMTRV TGLGCTATAL IGAFAAVEPD PLVAACAGLC ATGLAGELAA 

       250        260        270 
IRADGPGSLQ VEFLDALYAL DSAILRDAAR WVLP 

« Hide

References

[1]"Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis."
Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M.
J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1260 / CGDNIH1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000394 Genomic DNA. Translation: ABI61198.1. Different initiation.
RefSeqYP_744121.1. NC_008343.1.

3D structure databases

ProteinModelPortalQ0BVF4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391165.GbCGDNIH1_0300.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI61198; ABI61198; GbCGDNIH1_0300.
GeneID4274769.
KEGGgbe:GbCGDNIH1_0300.
PATRIC22076643. VBIGraBet83793_0317.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2145.
HOGENOMHOG000114352.
KOK00878.
OrthoDBEOG628F8M.

Enzyme and pathway databases

BioCycGBET391165:GHON-307-MONOMER.
UniPathwayUPA00060; UER00139.

Family and domain databases

HAMAPMF_00228. Thz_kinase.
InterProIPR000417. Hyethyz_kinase.
[Graphical view]
PfamPF02110. HK. 1 hit.
[Graphical view]
PIRSFPIRSF000513. Thz_kinase. 1 hit.
PRINTSPR01099. HYETHTZKNASE.
TIGRFAMsTIGR00694. thiM. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTHIM_GRABC
AccessionPrimary (citable) accession number: Q0BVF4
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: September 22, 2009
Last modified: February 19, 2014
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways