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Q0BV39

- Q0BV39_GRABC

UniProt

Q0BV39 - Q0BV39_GRABC

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciGBET391165:GHON-426-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:GbCGDNIH1_0415Imported
OrganismiGranulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)Imported
Taxonomic identifieri391165 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGranulibacter
ProteomesiUP000001963: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi391165.GbCGDNIH1_0415.

Structurei

3D structure databases

ProteinModelPortaliQ0BV39.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini520 – 631112HDUniRule annotationAdd
BLAST
Domaini756 – 83782ACT 1UniRule annotationAdd
BLAST
Domaini869 – 95183ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 390390UridylyltransferaseUniRule annotationAdd
BLAST
Regioni391 – 755365Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 1 HD domain.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains ACT domains.SAAS annotation
Contains HD domain.SAAS annotation

Keywords - Domaini

RepeatUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0BV39-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVTSSSPGLA QKAPRQTASE LAALELDRAL TALEEEFGPG PRKGPLSQLE
60 70 80 90 100
RNAALGVFRR HLARIQGRVR DTFEQGRLAG LTAAHLLSDL TDGLVAALYQ
110 120 130 140 150
YTDSIAIPAS ATQQTEPVTV AATGGYGRQE LAPFSDIDLL FITADTPSAR
160 170 180 190 200
TLAVVEFMLY FLWDLGLKVG HATRSISQSL QAATEDTTVR TTLLDTRHLA
210 220 230 240 250
GNQPLFDAFI TAFREACTEA GEHDFIAAKQ AERHARHLRY GESPFFVEPN
260 270 280 290 300
IKEGRGGLRD LQTLYWLSRY VFGTRKLVDL VGTGAPGGGL ITENEARLAK
310 320 330 340 350
RSWNFLWTLR FHLHYVAGRA EERLTFDVQP VIGARMGYTR HGRQNGVERF
360 370 380 390 400
MRHYFLTARE VLRLTRVLEP ALIRAALGPP AIAAETDIAL INAGFVLAEG
410 420 430 440 450
KLLPAPSHDF EREPIQMLRI MKLARDRDLQ IHPLGIRSLI RNERGAIALR
460 470 480 490 500
DDQRAAALFL DLLCGNTVPD GVQRPTQRPS GARWMSILNE TGFLGRFIPE
510 520 530 540 550
WARIVGQMQF DTYHVFTVDE HTIEAIRVLN QLEHGDLADI APVATGVVDH
560 570 580 590 600
LQSRRALYVA MLTHDIAKGR GGDHSELGAE LALEIGPALG LDSEETEMVS
610 620 630 640 650
WLVLHHLLLS QTAFRRDIDD PKTILDLADT IQSPERLKLL LVLTVADMRA
660 670 680 690 700
VSSKVWNGWK ATLLRELYAR VAEVLAGGLA TTERDTRVAR AKEAAAELLY
710 720 730 740 750
DWPEEQREAF LTLGYPGYWL SFDPETHARH ARMIRAAGTQ LLTVDTQPLP
760 770 780 790 800
ARAVTEVTVY VADTPGLVGK IAGALAVAGA SIVDARIHTM TNGMAMDTFW
810 820 830 840 850
VQDTSGEAFD QPNRLAKIAV LIEQALSGQL DIDEEIRKAS NPLLGTRMRA
860 870 880 890 900
IHVPPRVVVD NHASHTHTVL EVNGRDRPGL MHDIAAAIAQ QGLQIASAHI
910 920 930 940 950
TTYGVRAVDV FYVKDVFGLK VENERKLAKL RQALLGALTS PDDTGPMPLP
960
PPMRRTHATG DDF
Length:963
Mass (Da):106,130
Last modified:October 17, 2006 - v1
Checksum:iFACD7A2CBB1E00F1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000394 Genomic DNA. Translation: ABI61313.1.
RefSeqiWP_011631123.1. NC_008343.1.
YP_744236.1. NC_008343.1.

Genome annotation databases

EnsemblBacteriaiABI61313; ABI61313; GbCGDNIH1_0415.
GeneIDi4275199.
KEGGigbe:GbCGDNIH1_0415.
PATRICi22076889. VBIGraBet83793_0436.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000394 Genomic DNA. Translation: ABI61313.1 .
RefSeqi WP_011631123.1. NC_008343.1.
YP_744236.1. NC_008343.1.

3D structure databases

ProteinModelPortali Q0BV39.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 391165.GbCGDNIH1_0415.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI61313 ; ABI61313 ; GbCGDNIH1_0415 .
GeneIDi 4275199.
KEGGi gbe:GbCGDNIH1_0415.
PATRICi 22076889. VBIGraBet83793_0436.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci GBET391165:GHON-426-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis."
    Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M.
    J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1260 / CGDNIH1Imported.

Entry informationi

Entry nameiQ0BV39_GRABC
AccessioniPrimary (citable) accession number: Q0BV39
Entry historyi
Integrated into UniProtKB/TrEMBL: October 17, 2006
Last sequence update: October 17, 2006
Last modified: October 29, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotationSAAS annotation, Reference proteomeImported

External Data

Dasty 3