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Q0BV39

- Q0BV39_GRABC

UniProt

Q0BV39 - Q0BV39_GRABC

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotationSAAS annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    HydrolaseUniRule annotationSAAS annotation, NucleotidyltransferaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Ligandi

    MagnesiumUniRule annotationSAAS annotation

    Enzyme and pathway databases

    BioCyciGBET391165:GHON-426-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:GbCGDNIH1_0415Imported
    OrganismiGranulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)Imported
    Taxonomic identifieri391165 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGranulibacter
    ProteomesiUP000001963: Chromosome

    Interactioni

    Protein-protein interaction databases

    STRINGi391165.GbCGDNIH1_0415.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0BV39.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini520 – 631112HDUniRule annotationAdd
    BLAST
    Domaini756 – 83782ACT 1UniRule annotationAdd
    BLAST
    Domaini869 – 95183ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 390390UridylyltransferaseUniRule annotationAdd
    BLAST
    Regioni391 – 755365Uridylyl-removingUniRule annotationAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 1 HD domain.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains ACT domains.SAAS annotation
    Contains HD domain.SAAS annotation

    Keywords - Domaini

    RepeatUniRule annotationSAAS annotation

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261779.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0BV39-1 [UniParc]FASTAAdd to Basket

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    MVTSSSPGLA QKAPRQTASE LAALELDRAL TALEEEFGPG PRKGPLSQLE    50
    RNAALGVFRR HLARIQGRVR DTFEQGRLAG LTAAHLLSDL TDGLVAALYQ 100
    YTDSIAIPAS ATQQTEPVTV AATGGYGRQE LAPFSDIDLL FITADTPSAR 150
    TLAVVEFMLY FLWDLGLKVG HATRSISQSL QAATEDTTVR TTLLDTRHLA 200
    GNQPLFDAFI TAFREACTEA GEHDFIAAKQ AERHARHLRY GESPFFVEPN 250
    IKEGRGGLRD LQTLYWLSRY VFGTRKLVDL VGTGAPGGGL ITENEARLAK 300
    RSWNFLWTLR FHLHYVAGRA EERLTFDVQP VIGARMGYTR HGRQNGVERF 350
    MRHYFLTARE VLRLTRVLEP ALIRAALGPP AIAAETDIAL INAGFVLAEG 400
    KLLPAPSHDF EREPIQMLRI MKLARDRDLQ IHPLGIRSLI RNERGAIALR 450
    DDQRAAALFL DLLCGNTVPD GVQRPTQRPS GARWMSILNE TGFLGRFIPE 500
    WARIVGQMQF DTYHVFTVDE HTIEAIRVLN QLEHGDLADI APVATGVVDH 550
    LQSRRALYVA MLTHDIAKGR GGDHSELGAE LALEIGPALG LDSEETEMVS 600
    WLVLHHLLLS QTAFRRDIDD PKTILDLADT IQSPERLKLL LVLTVADMRA 650
    VSSKVWNGWK ATLLRELYAR VAEVLAGGLA TTERDTRVAR AKEAAAELLY 700
    DWPEEQREAF LTLGYPGYWL SFDPETHARH ARMIRAAGTQ LLTVDTQPLP 750
    ARAVTEVTVY VADTPGLVGK IAGALAVAGA SIVDARIHTM TNGMAMDTFW 800
    VQDTSGEAFD QPNRLAKIAV LIEQALSGQL DIDEEIRKAS NPLLGTRMRA 850
    IHVPPRVVVD NHASHTHTVL EVNGRDRPGL MHDIAAAIAQ QGLQIASAHI 900
    TTYGVRAVDV FYVKDVFGLK VENERKLAKL RQALLGALTS PDDTGPMPLP 950
    PPMRRTHATG DDF 963
    Length:963
    Mass (Da):106,130
    Last modified:October 17, 2006 - v1
    Checksum:iFACD7A2CBB1E00F1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000394 Genomic DNA. Translation: ABI61313.1.
    RefSeqiYP_744236.1. NC_008343.1.

    Genome annotation databases

    EnsemblBacteriaiABI61313; ABI61313; GbCGDNIH1_0415.
    GeneIDi4275199.
    KEGGigbe:GbCGDNIH1_0415.
    PATRICi22076889. VBIGraBet83793_0436.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000394 Genomic DNA. Translation: ABI61313.1 .
    RefSeqi YP_744236.1. NC_008343.1.

    3D structure databases

    ProteinModelPortali Q0BV39.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 391165.GbCGDNIH1_0415.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABI61313 ; ABI61313 ; GbCGDNIH1_0415 .
    GeneIDi 4275199.
    KEGGi gbe:GbCGDNIH1_0415.
    PATRICi 22076889. VBIGraBet83793_0436.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261779.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci GBET391165:GHON-426-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis."
      Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M.
      J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1260 / CGDNIH1Imported.

    Entry informationi

    Entry nameiQ0BV39_GRABC
    AccessioniPrimary (citable) accession number: Q0BV39
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 17, 2006
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzymeUniRule annotationSAAS annotation, Reference proteomeImported

    External Data

    Dasty 3