Q0BUK0 (ISPG_GRABC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
EC=1.17.7.1

Alternative name(s):
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Gene names

Name:	ispG
Ordered Locus Names:	GbCGDNIH1_0604

Organism

Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) [Complete proteome] [HAMAP]

Taxonomic identifier

391165 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Granulibacter › Granulibacter bethesdensis

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate By similarity. HAMAP-Rule MF_00159
Catalytic activity	(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin. HAMAP-Rule MF_00159
Cofactor	Binds 1 4Fe-4S cluster By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 5/6. HAMAP-Rule MF_00159
Sequence similarities	Belongs to the IspG family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase HAMAP-Rule MF_00159		PRO_1000203505
Sites
	Metal binding	272	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	275	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	307	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	314	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BUK0 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 23CB5FAB8A491F35	FASTA	387	41,473
	10 20 30 40 50 60 MSYRPYQQIE RRKSRQIHVG KVAVGGDAPI SVQTMTNTLT TDAEATIAQI RRAELAGVDI 70 80 90 100 110 120 VRVSCPDQES TIALKEIVRE VNVPIVADIH FHYRRAIEAA EAGAACLRIN PGNIGSAERV 130 140 150 160 170 180 REVVKAARDH GCAIRIGVNA GSLERHLLEK YGEPNPDALV ESALEHAKIL QDHDFHEFKI 190 200 210 220 230 240 SVKASDVFLA VAAYQQLAEV CDHPLHIGIT EAGGRRTGTV KSAIGLGNLL WAGVGDTMRV 250 260 270 280 290 300 SLSAEPEEEV HVGWEILKGL GLRHRGVKII SCPSCARQGF NVIETVAALE ERLAHIQTPL 310 320 330 340 350 360 TLSIIGCVVN GPGEALMTDI GLTGGGGGRH MIYNAGKTDH TIAADGMVDH IVTLVEEKAA 370 380 RIVEEEAAAK AAAEQAAQEA ALQHETV

References

[1]

"Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis." Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M. J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1260 / CGDNIH1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000394 Genomic DNA. Translation: ABI61502.1.
RefSeq	YP_744425.1. NC_008343.1.
3D structure databases
ProteinModelPortal	Q0BUK0.
ModBase	Search...
Protein-protein interaction databases
STRING	391165.GbCGDNIH1_0604.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI61502; ABI61502; GbCGDNIH1_0604.
GeneID	4276796.
KEGG	gbe:GbCGDNIH1_0604.
PATRIC	22077285. VBIGraBet83793_0633.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0821.
HOGENOM	HOG000261019.
KO	K03526.
OMA	PRKLEIV.
ProtClustDB	PRK00366.
Enzyme and pathway databases
BioCyc	GBET391165:GHON-665-MONOMER.
UniPathway	UPA00056; UER00096.
Family and domain databases
HAMAP	MF_00159. IspG.
InterPro	IPR016425. IspG_bac. IPR004588. IspG_bac-typ. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF04551. GcpE. 1 hit. [Graphical view]
PIRSF	PIRSF004640. IspG. 1 hit.
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00612. ispG_gcpE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPG_GRABC

Accession

Primary (citable) accession number: Q0BUK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program