Q0BUJ8 (RF1_GRABC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide chain release factor 1
Short name=RF-1

Gene names

Name:	prfA
Ordered Locus Names:	GbCGDNIH1_0606

Organism

Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) [Complete proteome] [HAMAP]

Taxonomic identifier

391165 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Granulibacter › Granulibacter bethesdensis

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA By similarity. HAMAP-Rule MF_00093
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Methylated by PrmC. Methylation increases the termination efficiency of RF1 By similarity. HAMAP-Rule MF_00093
Sequence similarities	Belongs to the prokaryotic/mitochondrial release factor family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
PTM	Methylation
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	translation release factor activity, codon specific Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Peptide chain release factor 1 HAMAP-Rule MF_00093		PRO_0000263281
Amino acid modifications
	Modified residue	229	1	N5-methylglutamine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BUJ8 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: A3816F05C06376CB	FASTA	352	39,011
	10 20 30 40 50 60 MSFTANLDRI VARAEELRAS LSEGLNGDSF AAASRELAEL EPVVARIEEL WAAERGLAEA 70 80 90 100 110 120 EAMLADPDMR ELAESEAEIL REKLPSLTRE IRIALLPKDE ADERSAILEI RPAAGGDEAS 130 140 150 160 170 180 LFAAQLFSMY QRYAEIRGWR FEILEYDDTG LGGLKGGMAE ITGRSVFARL KYESGVHRVQ 190 200 210 220 230 240 RVPATESQGR IHTSTVTVAV LPEAEDVDVQ VDEGDLRIDV YRASGAGGQH VNKTESAVRI 250 260 270 280 290 300 THLPSGIVVA MQEEKSQHKN RAKAMKILKA RLYEQQRAAL HASRAADRRA QVGTGDRSER 310 320 330 340 350 IRTYNFPQGR VSDHRINLTL YKIDRVMLGE LDDFVDALTA EDQAARLSAQ EL

References

[1]

"Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis." Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M. J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1260 / CGDNIH1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000394 Genomic DNA. Translation: ABI61504.1.
RefSeq	YP_744427.1. NC_008343.1.
3D structure databases
ProteinModelPortal	Q0BUJ8.
SMR	Q0BUJ8. Positions 89-348.
ModBase	Search...
Protein-protein interaction databases
STRING	391165.GbCGDNIH1_0606.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI61504; ABI61504; GbCGDNIH1_0606.
GeneID	4274965.
KEGG	gbe:GbCGDNIH1_0606.
PATRIC	22077289. VBIGraBet83793_0635.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0216.
HOGENOM	HOG000074815.
KO	K02835.
OMA	SVMLEIR.
ProtClustDB	PRK00591.
Enzyme and pathway databases
BioCyc	GBET391165:GHON-667-MONOMER.
Family and domain databases
Gene3D	3.30.160.20. 1 hit.
HAMAP	MF_00093. Rel_fac_1.
InterPro	IPR014720. dsRNA-bd-like_dom. IPR005139. PCRF. IPR000352. Pep_chain_release_fac_I_II. IPR004373. PrfA. [Graphical view]
Pfam	PF03462. PCRF. 1 hit. PF00472. RF-1. 1 hit. [Graphical view]
SMART	SM00937. PCRF. 1 hit. [Graphical view]
TIGRFAMs	TIGR00019. prfA. 1 hit.
PROSITE	PS00745. RF_PROK_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RF1_GRABC

Accession

Primary (citable) accession number: Q0BUJ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program