Q0BUD7 (Q0BUD7_GRABC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Cobyrinic acid A,C-diamide synthase HAMAP-Rule MF_00027

Gene names

Name:	cobB HAMAP-Rule MF_00027
Ordered Locus Names:	GbCGDNIH1_0667

Organism

Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) [Complete proteome] [HAMAP] EMBL ABI61565.1

Taxonomic identifier

391165 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Granulibacter › Granulibacter bethesdensis

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH2 groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation By similarity. HAMAP-Rule MF_00027
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis. HAMAP-Rule MF_00027
Sequence similarities	Belongs to the CobB/CobQ family. CobB subfamily. HAMAP-Rule MF_00027 Contains 1 GATase cobBQ-type domain. HAMAP-Rule MF_00027 SAAS SAAS011698

Ontologies

Keywords
Biological process	Cobalamin biosynthesis HAMAP-Rule MF_00027 SAAS SAAS011698
Domain	Glutamine amidotransferase HAMAP-Rule MF_00027 SAAS SAAS011698
Molecular function	Ligase EMBL ABI61565.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: HAMAP ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	354	1	Nucleophile By similarity HAMAP-Rule MF_00027
	Active site	457	1	By similarity HAMAP-Rule MF_00027

Sequences

	Sequence		Length	Mass (Da)
	Q0BUD7 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 8F704CBA7B810CB7	FASTA	464	48,338
	10 20 30 40 50 60 MSEGRRDEKG SGRAGQTGPG AAFMTAVKAL MVSAPKSGSG KTTVTLALAL ALRQRGVRVR 70 80 90 100 110 120 IAKNGPDYID PAFHAEAVGL PCFNLDSWAM PPAALDAIWT ELAAETDLIL IEASMGLFDG 130 140 150 160 170 180 GGSQPGRMGA ASDLADRFGV PVLLVLDVSG QSQSAAAVAR GFATHDPAVH LAGVIANNLA 190 200 210 220 230 240 SDRHAGGVAH AMQRAGLPLL GTVRRGAVPS LPERHLGLVQ AREHGTLPAL FEALANVAEA 250 260 270 280 290 300 SFDLDAILSA AVPVVLPPRE DSAPLLPPPG QRIALAWDDA FSFAYPHMLA GWRREGAEIV 310 320 330 340 350 360 CFSPLADQPP SEDCDAVWLP GGYPELHAAR LATASRFRSG MLRAAEQGAV HGECGGYMAL 370 380 390 400 410 420 GAGLEDAEGV IHPMLSLLSH VTSFRQRRLN LGYRQAILEA ASVLGAQGAI LRGHEFHYAR 430 440 450 460 VIDPGSDAPL ARLSDAEGCA LGLSGGIRGR VSGSFFHVLA REAA

References

[1]

"Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis." Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M. J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1260 / CGDNIH1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000394 Genomic DNA. Translation: ABI61565.1.
RefSeq	YP_744488.1. NC_008343.1.
3D structure databases
ProteinModelPortal	Q0BUD7.
ModBase	Search...
Protein-protein interaction databases
STRING	391165.GbCGDNIH1_0667.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI61565; ABI61565; GbCGDNIH1_0667.
GeneID	4275317.
KEGG	gbe:GbCGDNIH1_0667.
PATRIC	22077415. VBIGraBet83793_0697.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1797.
HOGENOM	HOG000289959.
KO	K02224.
OMA	CWLPGGY.
ProtClustDB	PRK01077.
Enzyme and pathway databases
BioCyc	GBET391165:GHON-728-MONOMER.
UniPathway	UPA00148.
Family and domain databases
HAMAP	MF_00027. CobB.
InterPro	IPR004484. CbiA_synth. IPR017929. CobB/CobQ_GATase. IPR002586. CobQ/CobB/MinD/ParA_Nub-bd_dom. IPR011698. GATase_3. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. PF07685. GATase_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00379. cobB. 1 hit.
PROSITE	PS51274. GATASE_COBBQ. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q0BUD7_GRABC

Accession

Primary (citable) accession number: Q0BUD7

Entry history

Integrated into UniProtKB/TrEMBL:	October 17, 2006
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)