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Q0BU54 (GLGB_GRABC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:GbCGDNIH1_0750
OrganismGranulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) [Complete proteome] [HAMAP]
Taxonomic identifier391165 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGranulibacter

Protein attributes

Sequence length734 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7347341,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000260659

Sites

Active site4131Nucleophile By similarity
Active site4661Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0BU54 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 902B363512AED48E

FASTA73482,302
        10         20         30         40         50         60 
MNALDPDTLI HPDVATAIIH GRHGDPFSVL GIHTTREGVV LRSFQPEATS VVAINPESGQ 

        70         80         90        100        110        120 
EIATLHRVHG DGIFAARLPG RTGYRLKIVT ATGTDIIDDP YVFPNLLGDL DVYLLAEGRH 

       130        140        150        160        170        180 
EDLGRVLGAH VTTMHSPDGR EVEGVRFAVW APNATRASVI GSFNHWDGRC HPMRKRVEAG 

       190        200        210        220        230        240 
VWELFIPGLT AGTIYKYELL GPNGELLPLK ADPVAWYAEI PPATASIVAS PAPFQWTDEQ 

       250        260        270        280        290        300 
WMQQRGRLQA ADQPISIYEV HPTSWMPAKC GDWNTLADRL IPYVQSLGFT HVEFLPVMEH 

       310        320        330        340        350        360 
PFGGSWGYQP LGQFAPMARL GTPESFASLV NRLHEVGIGV ILDWVPAHFP TDAHGLARFD 

       370        380        390        400        410        420 
GTALYEHEDP REGYHYDWNT YIYNLGRNEV RAFLISSAVH WLTYFHADAL RVDAVASMLY 

       430        440        450        460        470        480 
RDYSRKHGEW IPNMYGGREN LESVSFLQHL STVVKSRAPG AVLIAEESTA WPGVTRPAHE 

       490        500        510        520        530        540 
GGLGFDYKWN MGWMHDTLHY MQEDPVNRSW HHDQMTFGLV YAFSEKFVLP LSHDEVVHGK 

       550        560        570        580        590        600 
RSLLGRMPGD EWQRFANLRA YYGFMWTHPG KKLLFMGGEI AQEQEWSEGH GTDWYLLDRP 

       610        620        630        640        650        660 
NHQGVQRLVA DLNMLYRAEP ALYRKDCAWD GFSWLVIDDR TQSVFAYMRY GADSDAPVLV 

       670        680        690        700        710        720 
VCNLTPVPRH DYRIGVPQGG YWREILNTDA GIYGGSNTGN GGGRMADATP SHHHPASLQL 

       730 
TLPPLATIIL KPES 

« Hide

References

[1]"Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis."
Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M.
J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1260 / CGDNIH1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000394 Genomic DNA. Translation: ABI61648.1.
RefSeqYP_744571.1. NC_008343.1.

3D structure databases

ProteinModelPortalQ0BU54.
SMRQ0BU54. Positions 123-734.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING391165.GbCGDNIH1_0750.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI61648; ABI61648; GbCGDNIH1_0750.
GeneID4274989.
KEGGgbe:GbCGDNIH1_0750.
PATRIC22077603. VBIGraBet83793_0784.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycGBET391165:GHON-771-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_GRABC
AccessionPrimary (citable) accession number: Q0BU54
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: October 17, 2006
Last modified: June 11, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries