Reviewed, UniProtKB/Swiss-Prot Q0BTZ0 (SYE1_GRABC)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	GbCGDNIH1_0814

Organism

Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) [Complete proteome] [HAMAP]

Taxonomic identifier

391165 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Granulibacter

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000330973
Regions
	Motif	23 – 33	11	"HIGH" region HAMAP MF_00022
	Motif	252 – 256	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	255	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BTZ0-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 5081EECA24D166E8	FASTA	473	52,327
	10 20 30 40 50 60 MHSLTTHKHV RGGDMTIRTR FAPSPTGLLH VGGARTALFN YLFARHHGGE YLLRIEDTDR 70 80 90 100 110 120 ARSTPEAVQV ILDGLDWLGL SPDQEPVFQS TRETRHTEVA HSLLERGMAY RCFLSPEELQ 130 140 150 160 170 180 AQRDQAAADK RPLRINSPWR DRDLSEAPSG IAPVIRLRVP QEGETVVDDL VQGPVRVANA 190 200 210 220 230 240 ELDDMIILRS DGTPTYLHAV VVDDHDMAIT HVIRGDDHLT NTFRQVQIFK ALGWDLPRFA 250 260 270 280 290 300 HIPLIHGADG AKLSKRHGAV SVLEFEREGF LPEALCNYLL RLGWGHGDAE ILSRDEQVAL 310 320 330 340 350 360 FDLDGVGRAA SRMDYAKLTH LNGVYLREAD DQRLTTDLCR RLDLSPDSDA SSRIRRLMPK 370 380 390 400 410 420 LKERARTLVD LAESARFVIE RPSGPKDAKA AALLSDEARG WLRALLPQLQ ATDFSAAAVD 430 440 450 460 470 QALRAFAEEH GLKLGQIAQP LRVALTGGTT SPPIDATLEA LGASEVTSRI EAV

References

[1]

"Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis." Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M. J. Bacteriol. 189:8727-8736(2007) [PubMed: 17827295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000394 Genomic DNA. Translation: ABI61712.1.
RefSeq	YP_744635.1.
3D structure databases
SMR	Q0BTZ0. Positions 17-472.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BTZ0.
Genome annotation databases
GeneID	4275090.
GenomeReviews	Gene locus GbCGDNIH1_0814 in contig CP000394_GR.
KEGG	gbe:GbCGDNIH1_0814.
NMPDR	fig|391165.8.peg.819.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	MAHIPLI.
PhylomeDB	Q0BTZ0.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_GRABC

Accession

Primary (citable) accession number: Q0BTZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	October 17, 2006
Last modified:	February 9, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)