Q0BTX5 (KDSA_GRABC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydro-3-deoxyphosphooctonate aldolase
EC=2.5.1.55

Alternative name(s):
3-deoxy-D-manno-octulosonic acid 8-phosphate synthase
KDO-8-phosphate synthase
Short name=KDO 8-P synthase
Short name=KDOPS
Phospho-2-dehydro-3-deoxyoctonate aldolase

Gene names

Name:	kdsA
Ordered Locus Names:	GbCGDNIH1_0829

Organism

Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) [Complete proteome] [HAMAP]

Taxonomic identifier

391165 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Granulibacter

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the KdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_1000117784

Sequences

	Sequence		Length	Mass (Da)
	Q0BTX5 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 1C2FC586F5A3A08E	FASTA	282	30,081
	10 20 30 40 50 60 MSDRGENGQA RIVTVGNVRF GNDLPFVLIA GPCQIESRAH AEGVAAELHA ICARLDIPLV 70 80 90 100 110 120 FKASYDKANR TSVGGARGVG MERGLDILAG IRERFDIPVL TDIHDIGQCK PAAEAVDILQ 130 140 150 160 170 180 IPAFLCRQTD LLLAAGETGR TINVKKGQFL APWDMRNVAA KIASTGNHNL LLTERGASFG 190 200 210 220 230 240 YNTLVTDFRA LPIMAQTGYP VVFDATHSVQ QPGGQGTASG GDRTFAPILA RAALAVGVAS 250 260 270 280 VFIECHPDPD RAPSDGPNMI PLDQMEETLR RLKQFDVLGK AG

References

[1]

"Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis." Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M. J. Bacteriol. 189:8727-8736(2007) [PubMed: 17827295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1260 / CGDNIH1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000394 Genomic DNA. Translation: ABI61727.1.
RefSeq	YP_744650.1. NC_008343.1.
3D structure databases
ProteinModelPortal	Q0BTX5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BTX5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4275700.
GenomeReviews	Gene locus GbCGDNIH1_0829 in contig CP000394_GR.
KEGG	gbe:GbCGDNIH1_0829.
NMPDR	fig|391165.8.peg.834.
PATRIC	22077765. VBIGraBet83793_0865.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2877.
HOGENOM	HBG575990.
OMA	ITERGAM.
PhylomeDB	Q0BTX5.
ProtClustDB	PRK05198.
Enzyme and pathway databases
BioCyc	GBET391165:GBCGDNIH1_0829-MONOMER.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01627.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_GRABC

Accession

Primary (citable) accession number: Q0BTX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program