Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q0BSW6

- LIPA_GRABC

UniProt

Q0BSW6 - LIPA_GRABC

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Lipoyl synthase

Gene

lipA

Organism
Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi76 – 761Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi91 – 911Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi95 – 951Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi98 – 981Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciGBET391165:GHON-1222-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:GbCGDNIH1_1188
OrganismiGranulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)
Taxonomic identifieri391165 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGranulibacter
ProteomesiUP000001963: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Lipoyl synthasePRO_0000325260Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi391165.GbCGDNIH1_1188.

Structurei

3D structure databases

ProteinModelPortaliQ0BSW6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0BSW6 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVTRVLIDHR QGGRHEAGVR HPEKQHRPDN PSQPKPSWIR VKAPNHPVYH
60 70 80 90 100
QTQALMRENR LTTVCEEAAC PNIGECWSQR HATMMIMGDT CTRACSFCNV
110 120 130 140 150
KTGLPHALDG DEPARVADAV ARLGLRHVVI TSVDRDDLND GGAAHIAAVI
160 170 180 190 200
KAVRLAAPET TIEVLTPDFL RKPGAAETVA EARPDVFNHN LETVPRLYPA
210 220 230 240 250
IRPGARYFQS LRLLDQVKRL DPSIFTKSGL MVGLGEDRAE IAQVMDDLRI
260 270 280 290 300
AEVDFITLGQ YLQPTPKHAA VDRFVTPDEF SDYASMARSK GFLMVSSSPL
310 320
TRSSYHADAD FAALRAARME QQQRIA
Length:326
Mass (Da):36,155
Last modified:March 18, 2008 - v2
Checksum:iBC1E5767EDC32BFB
GO

Sequence cautioni

The sequence ABI62086.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000394 Genomic DNA. Translation: ABI62086.1. Different initiation.
RefSeqiYP_745009.1. NC_008343.1.

Genome annotation databases

EnsemblBacteriaiABI62086; ABI62086; GbCGDNIH1_1188.
GeneIDi4275734.
KEGGigbe:GbCGDNIH1_1188.
PATRICi22078535. VBIGraBet83793_1237.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000394 Genomic DNA. Translation: ABI62086.1 . Different initiation.
RefSeqi YP_745009.1. NC_008343.1.

3D structure databases

ProteinModelPortali Q0BSW6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 391165.GbCGDNIH1_1188.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI62086 ; ABI62086 ; GbCGDNIH1_1188 .
GeneIDi 4275734.
KEGGi gbe:GbCGDNIH1_1188.
PATRICi 22078535. VBIGraBet83793_1237.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci GBET391165:GHON-1222-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis."
    Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M.
    J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1260 / CGDNIH1.

Entry informationi

Entry nameiLIPA_GRABC
AccessioniPrimary (citable) accession number: Q0BSW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: October 29, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3