Q0BRW8 (GMHA_GRABC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoheptose isomerase
EC=5.3.1.28

Alternative name(s):
Sedoheptulose 7-phosphate isomerase

Gene names

Name:	gmhA
Ordered Locus Names:	GbCGDNIH1_1536

Organism

Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) [Complete proteome] [HAMAP]

Taxonomic identifier

391165 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Granulibacter › Granulibacter bethesdensis

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate By similarity. HAMAP-Rule MF_00067
Catalytic activity	D-sedoheptulose 7-phosphate = D-glycero-D-manno-heptose 7-phosphate. HAMAP-Rule MF_00067
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1. HAMAP-Rule MF_00067
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate By similarity. HAMAP-Rule MF_00067
Sequence similarities	Belongs to the SIS family. GmhA subfamily. Contains 1 SIS domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	D-glycero-D-manno-heptose 7-phosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway lipopolysaccharide core region biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	D-sedoheptulose 7-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP carbohydrate binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	Phosphoheptose isomerase HAMAP-Rule MF_00067		PRO_1000057452
Regions
	Domain	38 – 197	160	SIS
	Region	53 – 55	3	Substrate binding By similarity
	Region	95 – 96	2	Substrate binding By similarity
	Region	121 – 123	3	Substrate binding By similarity
Sites
	Metal binding	62	1	Zinc By similarity
	Metal binding	66	1	Zinc By similarity
	Metal binding	173	1	Zinc By similarity
	Metal binding	181	1	Zinc By similarity
	Binding site	66	1	Substrate By similarity
	Binding site	126	1	Substrate By similarity
	Binding site	173	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BRW8 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 8A229B9DB1047A1F	FASTA	197	21,065
	10 20 30 40 50 60 MDHRDFLTRF LDASCEAMDA FAADEAARET LIRMGEDVCT SIRNGGKLLI AGNGGSAADA 70 80 90 100 110 120 QHIAAEFVSR LMYDRAPLPA LALTTDSSAL TAIGNDYGYE HVFERQVTGL GQKGDVFLGI 130 140 150 160 170 180 STSGRSPNVI RALHAAREKG IITFGFTGVA GNAMTELCDR LLLAPSPQTA IIQQIHITAA 190 HMLCAVVERT LFPVEGR

References

[1]

"Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis." Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M. J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-1260 / CGDNIH1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000394 Genomic DNA. Translation: ABI62434.1.
RefSeq	YP_745357.1. NC_008343.1.
3D structure databases
ProteinModelPortal	Q0BRW8.
ModBase	Search...
Protein-protein interaction databases
STRING	391165.GbCGDNIH1_1536.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI62434; ABI62434; GbCGDNIH1_1536.
GeneID	4276133.
KEGG	gbe:GbCGDNIH1_1536.
PATRIC	22079253. VBIGraBet83793_1587.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0279.
HOGENOM	HOG000237571.
KO	K03271.
OMA	HAICDEI.
ProtClustDB	PRK13937.
Enzyme and pathway databases
BioCyc	GBET391165:GHON-1592-MONOMER.
UniPathway	UPA00041; UER00436.
Family and domain databases
HAMAP	MF_00067. GmhA.
InterPro	IPR004515. Phosphoheptose_Isoase. IPR001347. SIS. [Graphical view]
Pfam	PF13580. SIS_2. 1 hit. [Graphical view]
PROSITE	PS51464. SIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GMHA_GRABC

Accession

Primary (citable) accession number: Q0BRW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program