Q0BQD3 (Q0BQD3_GRABC) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malate dehydrogenase HAMAP MF_00487
EC=1.1.1.37 HAMAP MF_00487

Gene names

Name:	mdh HAMAP MF_00487
Ordered Locus Names:	GbCGDNIH1_2071

Organism

Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) [Complete proteome] [HAMAP] EMBL ABI62969.1

Taxonomic identifier

391165 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Granulibacter

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family. HAMAP MF_00487

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle HAMAP MF_00487
Ligand	NAD HAMAP MF_00487
Molecular function	Oxidoreductase HAMAP MF_00487 EMBL ABI62969.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	17 – 22	6	NAD By similarity HAMAP MF_00487
	Nucleotide binding	126 – 128	3	NAD By similarity HAMAP MF_00487
Sites
	Active site	183	1	Proton acceptor By similarity HAMAP MF_00487
	Binding site	41	1	NAD By similarity HAMAP MF_00487
	Binding site	90	1	Substrate By similarity HAMAP MF_00487
	Binding site	96	1	Substrate By similarity HAMAP MF_00487
	Binding site	103	1	NAD By similarity HAMAP MF_00487
	Binding site	128	1	Substrate By similarity HAMAP MF_00487
	Binding site	159	1	Substrate By similarity HAMAP MF_00487

Sequences

	Sequence		Length	Mass (Da)
	Q0BQD3 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 6ADF4809D704484E	FASTA	324	33,898
	10 20 30 40 50 60 MPSEGDPMAR NKIALIGAGH IGGTLAHLIG LKELGDVVLF DVFGGVAAGK ALDIMQSAPV 70 80 90 100 110 120 DGFDSTMVGG SDYAAIAGAD VVIVTAGFPR MPGMSRDDLV AKNAEVIAQV AEGIRTHAPD 130 140 150 160 170 180 AFVIVITNPL DAMVWVMQQK SGLPPHKVVG MAGVLDSARF RLFLAHEFNV SVEDVTAFVL 190 200 210 220 230 240 GGHGDTMVPL IRYSTVAGIP VPDLIRMGWT TQEKIDAIVS RTANGGGEIV KLLEKGSAFY 250 260 270 280 290 300 APAASAIAMT ESYLKDKKRV MPCAAHLSGQ YGIDGLYVGV PVVIGAGGVE RVVEISLNEQ 310 320 EQAAFDKSCD SVKELIAASR KLIG

References

[1]

"Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis." Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M. J. Bacteriol. 189:8727-8736(2007) [PubMed: 17827295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000394 Genomic DNA. Translation: ABI62969.1.
RefSeq	YP_745892.1. NC_008343.1.
3D structure databases
ProteinModelPortal	Q0BQD3.
SMR	Q0BQD3. Positions 12-317.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BQD3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4275567.
GenomeReviews	Gene locus GbCGDNIH1_2071 in contig CP000394_GR.
KEGG	gbe:GbCGDNIH1_2071.
NMPDR	fig|391165.8.peg.2012.
PATRIC	22080401. VBIGraBet83793_2149.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HBG566126.
OMA	NGRNRIL.
PhylomeDB	Q0BQD3.
Family and domain databases
HAMAP	MF_00487. Malate_dehydrog_3. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR011275. Malate_DH_type3. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00024.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01763. MalateDH_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q0BQD3_GRABC

Accession

Primary (citable) accession number: Q0BQD3

Entry history

Integrated into UniProtKB/TrEMBL:	October 17, 2006
Last sequence update:	October 17, 2006
Last modified:	December 14, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)