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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway:iN(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei54 – 541S-adenosyl-L-methionineUniRule annotation
Binding sitei79 – 791S-adenosyl-L-methionineUniRule annotation
Binding sitei114 – 1141S-adenosyl-L-methionineUniRule annotation
Active sitei136 – 1361By similarity
Binding sitei136 – 1361S-adenosyl-L-methionineUniRule annotation
Binding sitei140 – 1401SubstrateUniRule annotation
Binding sitei172 – 1721SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciGBET391165:GHON-2455-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:GbCGDNIH1_2398
OrganismiGranulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1)
Taxonomic identifieri391165 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGranulibacter
ProteomesiUP000001963 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227tRNA (guanine-N(7)-)-methyltransferasePRO_0000288153Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi391165.GbCGDNIH1_2398.

Structurei

3D structure databases

ProteinModelPortaliQ0BPF6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni206 – 2094Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OMAiIGCEPFV.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0BPF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPDMTMKSQP DRLYGRQRGH ALRPRQQRLL DLTLPRLRYA GPSSLRGVSP
60 70 80 90 100
LWLEIGFGGG EHAVAQIEAH PDVTLIACEV FENGLCSLLS RLLPEPLDEE
110 120 130 140 150
TAPLPGNLRV WDDDARPLLR DLPDQVLDRV FLMFPDPWPK ARHAKRRFVH
160 170 180 190 200
PENAATLARV MKPGAEWRIA SDDPTYQAWV PEVMERQTAF ELLAVSNERP
210 220
EGWPPTRYEA KAIRAGRQPL YWRYVRR
Length:227
Mass (Da):26,101
Last modified:October 17, 2006 - v1
Checksum:iD750EDD5F2BA84BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000394 Genomic DNA. Translation: ABI63296.1.
RefSeqiWP_011633098.1. NC_008343.1.

Genome annotation databases

EnsemblBacteriaiABI63296; ABI63296; GbCGDNIH1_2398.
KEGGigbe:GbCGDNIH1_2398.
PATRICi22081099. VBIGraBet83793_2491.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000394 Genomic DNA. Translation: ABI63296.1.
RefSeqiWP_011633098.1. NC_008343.1.

3D structure databases

ProteinModelPortaliQ0BPF6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391165.GbCGDNIH1_2398.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI63296; ABI63296; GbCGDNIH1_2398.
KEGGigbe:GbCGDNIH1_2398.
PATRICi22081099. VBIGraBet83793_2491.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OMAiIGCEPFV.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciGBET391165:GHON-2455-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence analysis of the emerging human pathogenic acetic acid bacterium Granulibacter bethesdensis."
    Greenberg D.E., Porcella S.F., Zelazny A.M., Virtaneva K., Sturdevant D.E., Kupko J.J. III, Barbian K.D., Babar A., Dorward D.W., Holland S.M.
    J. Bacteriol. 189:8727-8736(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1260 / CGDNIH1.

Entry informationi

Entry nameiTRMB_GRABC
AccessioniPrimary (citable) accession number: Q0BPF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: October 17, 2006
Last modified: July 22, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.