Q0BNB6 (LSPA_FRATO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	FTH_0427

Organism

Francisella tularensis subsp. holarctica (strain OSU18) [Complete proteome] [HAMAP]

Taxonomic identifier

393011 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella › Francisella tularensis › Francisella tularensis subsp. holarctica

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP-Rule MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP-Rule MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP-Rule MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	Lipoprotein signal peptidase HAMAP-Rule MF_00161		PRO_0000289380
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	40 – 60	21	Helical; Potential
	Transmembrane	67 – 87	21	Helical; Potential
	Transmembrane	91 – 111	21	Helical; Potential
	Transmembrane	136 – 156	21	Helical; Potential
Sites
	Active site	113	1	By similarity
	Active site	140	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BNB6 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: F3E5194B95D253FB	FASTA	161	18,181
	10 20 30 40 50 60 MNLLRPKLKY FILAILIIAA DLYTKYLANT YLEFAQSLKI TSFFNLTLLY NHGAAFSLLS 70 80 90 100 110 120 NDQTSWQMIM FSTISLIAAI VLIYLIIKQP ITEKINLFSF ALILGGALGN FYDRAFQGYV 130 140 150 160 IDFLDFHIGN YHWPSFNIAD SAITCGVVIL IAASLFTKKK S

References

[1]

"Chromosome rearrangement and diversification of Francisella tularensis revealed by the type B (OSU18) genome sequence." Petrosino J.F., Xiang Q., Karpathy S.E., Jiang H., Yerrapragada S., Liu Y., Gioia J., Hemphill L., Gonzalez A., Raghavan T.M., Uzman A., Fox G.E., Highlander S., Reichard M., Morton R.J., Clinkenbeard K.D., Weinstock G.M. J. Bacteriol. 188:6977-6985(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: OSU18.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000437 Genomic DNA. Translation: ABI82418.1.
RefSeq	YP_763055.1. NC_008369.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	393011.FTH_0427.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABI82418; ABI82418; FTH_0427.
GeneID	4306475.
KEGG	fth:FTH_0427.
PATRIC	17950654. VBIFraTul3936_0489.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0597.
HOGENOM	HOG000096992.
KO	K03101.
OMA	DMSQRPN.
ProtClustDB	PRK00376.
Enzyme and pathway databases
BioCyc	FTUL393011:GHFN-696-MONOMER.
UniPathway	UPA00665.
Family and domain databases
HAMAP	MF_00161. LspA.
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_FRATO

Accession

Primary (citable) accession number: Q0BNB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program