Reviewed, UniProtKB/Swiss-Prot Q0BLD2 (BIOD_FRATO)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	FTH_1248

Organism

Francisella tularensis subsp. holarctica (strain OSU18) [Complete proteome] [HAMAP]

Taxonomic identifier

393011 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 6-carboxyhexanoyl-CoA: step 3/4. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	Dethiobiotin synthetase HAMAP MF_00336		PRO_0000302506
Regions
	Nucleotide binding	8 – 16	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BLD2-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 090B0BF3DAD24657	FASTA	219	24,502
	10 20 30 40 50 60 MKKFFIIGTN TEVGKTYIST KLIEVCEHQN IKSLCLKPVA SGQSQFSELC EDVESILNAY 70 80 90 100 110 120 KHKFTAAEIN LISFNQAVAP HIIAAKTKVD ISIENLKQFI EDKYNQDLDI LFIEGAGGLL 130 140 150 160 170 180 TPYSDHTTQL DLIKALQIPV LLVSAIKVGC INHTLLTINE LNRHNIKLAG WIANCNDSNI 190 200 210 KYIDEQINTI EELSGYKCSA KISRNADYLD FIDLSKILI

References

[1]

"Chromosome rearrangement and diversification of Francisella tularensis revealed by the type B (OSU18) genome sequence." Petrosino J.F., Xiang Q., Karpathy S.E., Jiang H., Yerrapragada S., Liu Y., Gioia J., Hemphill L., Gonzalez A., Raghavan T.M., Uzman A., Fox G.E., Highlander S., Reichard M., Morton R.J., Clinkenbeard K.D., Weinstock G.M. J. Bacteriol. 188:6977-6985(2006) [PubMed: 16980500] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000437 Genomic DNA. Translation: ABI83102.1.
RefSeq	YP_763739.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4307534.
GenomeReviews	Gene locus FTH_1248 in contig CP000437_GR.
KEGG	fth:FTH_1248.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q0BLD2.
OMA	Q0BLD2. CLIEGAG.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_FRATO

Accession

Primary (citable) accession number: Q0BLD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)