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Q0BK01 (DDL_FRATO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:FTH_1833
OrganismFrancisella tularensis subsp. holarctica (strain OSU18) [Complete proteome] [HAMAP]
Taxonomic identifier393011 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 296296D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341096

Regions

Domain103 – 293191ATP-grasp
Nucleotide binding129 – 18052ATP By similarity

Sites

Metal binding2471Magnesium or manganese 1 By similarity
Metal binding2601Magnesium or manganese 1 By similarity
Metal binding2601Magnesium or manganese 2 By similarity
Metal binding2621Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0BK01 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 8733294084172AA2

FASTA29632,772
        10         20         30         40         50         60 
MKNEKIVVLY GGDSPEREVS LKSGKAVLDS LISQGYDAVG VDASGKELVA KLLELKPDKC 

        70         80         90        100        110        120 
FVALHGEDGE NGRVSALLEM LEIKHTSSSM KSSVITMDKM ISKEILMHHR MPTPMAKFLT 

       130        140        150        160        170        180 
DKLVAEDEIS FPVAVKPSSG GSSIATFKVK SIQELKHAYE EASKYGEVMI EQWVTGKEIT 

       190        200        210        220        230        240 
VAIVNDEVYS SVWIEPQNEF YDYESKYSGK SIYHSPSGLC EQKELEVRQL AKKAYDLLGC 

       250        260        270        280        290 
SGHARVDFIY DDRGNFYIME INSSPGMTDN SLSPKSAAAE GVDFDSFVKR IIEQAQ 

« Hide

References

[1]"Chromosome rearrangement and diversification of Francisella tularensis revealed by the type B (OSU18) genome sequence."
Petrosino J.F., Xiang Q., Karpathy S.E., Jiang H., Yerrapragada S., Liu Y., Gioia J., Hemphill L., Gonzalez A., Raghavan T.M., Uzman A., Fox G.E., Highlander S., Reichard M., Morton R.J., Clinkenbeard K.D., Weinstock G.M.
J. Bacteriol. 188:6977-6985(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: OSU18.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000437 Genomic DNA. Translation: ABI83583.1.
RefSeqYP_764220.1. NC_008369.1.

3D structure databases

ProteinModelPortalQ0BK01.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING393011.FTH_1833.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI83583; ABI83583; FTH_1833.
GeneID4306615.
KEGGfth:FTH_1833.
PATRIC17953991. VBIFraTul3936_2110.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK14569.

Enzyme and pathway databases

BioCycFTUL393011:GHFN-1878-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_FRATO
AccessionPrimary (citable) accession number: Q0BK01
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: October 17, 2006
Last modified: February 19, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways