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Protein

Ribosomal RNA small subunit methyltransferase G

Gene

rsmG

Organism
Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Specifically methylates the N7 position of guanine in position 527 of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(527) in 16S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(527) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei94 – 941S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei159 – 1591S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBAMB339670:GH48-87-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase GUniRule annotation (EC:2.1.1.170UniRule annotation)
Alternative name(s):
16S rRNA 7-methylguanosine methyltransferaseUniRule annotation
Short name:
16S rRNA m7G methyltransferaseUniRule annotation
Gene namesi
Name:rsmGUniRule annotation
Ordered Locus Names:Bamb_0085
OrganismiBurkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD))
Taxonomic identifieri339670 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000000662 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 228228Ribosomal RNA small subunit methyltransferase GPRO_0000335317Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi339670.Bamb_0085.

Structurei

3D structure databases

ProteinModelPortaliQ0BJM7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni140 – 1412S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RNA methyltransferase RsmG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D28. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221012.
KOiK03501.
OMAiMVKHILD.
OrthoDBiPOG091H00IF.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G. 1 hit.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q0BJM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTARRAPAVN RDVLEQMLVD GTAALDIALT DAQRNQLLDY VALLGKWNAV
60 70 80 90 100
YNLTAIRDPK QMLIQHILDS LSIVPHLRDR ASARVLDVGS GGGLPGIVLA
110 120 130 140 150
IVRPDWQVTL NDIVQKKSAF QTQMRAELKL ANLSVVTGRV ELLQPGVDVP
160 170 180 190 200
EKFDMIVSRA FADLSDFVKL ARHLVAPGGS IWAMKGVHPD DEIARLPEGS
210 220
RVKQTMRLAV PMLDAERHLI EVIVDEAN
Length:228
Mass (Da):25,090
Last modified:October 17, 2006 - v1
Checksum:iC4A95765E59EEA2B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000440 Genomic DNA. Translation: ABI85646.1.
RefSeqiWP_011655612.1. NZ_CP009798.1.

Genome annotation databases

EnsemblBacteriaiABI85646; ABI85646; Bamb_0085.
KEGGibam:Bamb_0085.
PATRICi19015614. VBIBurAmb61564_0135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000440 Genomic DNA. Translation: ABI85646.1.
RefSeqiWP_011655612.1. NZ_CP009798.1.

3D structure databases

ProteinModelPortaliQ0BJM7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi339670.Bamb_0085.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI85646; ABI85646; Bamb_0085.
KEGGibam:Bamb_0085.
PATRICi19015614. VBIBurAmb61564_0135.

Phylogenomic databases

eggNOGiENOG4105D28. Bacteria.
COG0357. LUCA.
HOGENOMiHOG000221012.
KOiK03501.
OMAiMVKHILD.
OrthoDBiPOG091H00IF.

Enzyme and pathway databases

BioCyciBAMB339670:GH48-87-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00074. 16SrRNA_methyltr_G. 1 hit.
InterProiIPR003682. rRNA_ssu_MeTfrase_G.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02527. GidB. 1 hit.
[Graphical view]
PIRSFiPIRSF003078. GidB. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00138. rsmG_gidB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSMG_BURCM
AccessioniPrimary (citable) accession number: Q0BJM7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 17, 2006
Last modified: September 7, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.