Reviewed, UniProtKB/Swiss-Prot Q0BIK3 (MURD_BURCM)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	Bamb_0461

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000301418
Regions
	Nucleotide binding	129 – 135	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0BIK3-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 5852F862D56A00FB	FASTA	502	52,794
	10 20 30 40 50 60 MFGDRQRPMV LVLGLGESGL AIARWCARHG CRLRIADTRE APPNLAALQA EGIDAEFVGG 70 80 90 100 110 120 PFTPALLDGG VEIVGLSPGL SPLEPALAAL IAAANERAIA VWGELEFFAQ ALRALGTSGY 130 140 150 160 170 180 QPKVLAITGT NGKTTTTNLT GLLCQRSGKK VAVAGNISPA MLDRLARAID ETALPDVWVL 190 200 210 220 230 240 ELSSFQLETA RTFAPDAAAI LNITQDHLDW HGSFDAYAAA KGRIFGATTT RVLNRDDAAV 250 260 270 280 290 300 MKFAPAAGAA DAARTVTFGL NEPAQQGDYG LSRDNGIAWL VEAVDRDAPD ETTSRRRKRD 310 320 330 340 350 360 GAHTPDIAQK RLMPADALRI RGLHNAANAL AAFALARAID LPAAPLLHAL REYRGEAHRV 370 380 390 400 410 420 EVIATIDDVD YVDDSKGTNV GATVAALDGL AQKIVLIAGG DGKGQDFAPL VAPVARWCRA 430 440 450 460 470 480 VMLIGRDAPA IRDTLAETGV PLADHATLEA AVHAAAELAE PGDAVLLSPA CASLDMFRNY 490 500 AHRADVFRAA VDEIAIDKGA TT

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000440 Genomic DNA. Translation: ABI86020.1.
RefSeq	YP_772354.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4311150.
GenomeReviews	Gene locus Bamb_0461 in contig CP000440_GR.
KEGG	bam:Bamb_0461.
NMPDR	fig|339670.3.peg.6104.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q0BIK3. SEDHLDR.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BURCM

Accession

Primary (citable) accession number: Q0BIK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)