Reviewed, UniProtKB/Swiss-Prot Q0BIK0 (MURC_BURCM)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	Bamb_0464

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 465	465	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000004319
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q0BIK0-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 9F5985295DCC78E6	FASTA	465	48,979
	10 20 30 40 50 60 MKHIVKQIHF VGIGGAGMSG IAEVLVNLGY EVSGSDLSRN AVTDRLEALG ARIAIGHDAA 70 80 90 100 110 120 NIAGANAVVV STAVRSDNPE VLAARHQGVP IVQRAVMLAE LMRLKQGIAI AGTHGKTTTT 130 140 150 160 170 180 SLVASVLAAG GLDPTFVIGG RLISAGANAR LGTGDFIVAE ADESDASFLN LYPVIEVITN 190 200 210 220 230 240 IDADHMDTYG HDFARLKQAF IEFTQRLPFY GSAVVCVDDP NVRQIIPFIS KPVVRYGLSP 250 260 270 280 290 300 DAQVRAEDID ARDGRMHFTV IREGRAPLAV VLNLPGLHNV QNALAAIAIA TDLGVSDDAI 310 320 330 340 350 360 QQALAEFNGV GRRFQRYGEV PSADGGQYTL IDDYGHHPVE MAATVAAARG AFPGRRLVLA 370 380 390 400 410 420 FQPHRYTRTR DCFDDFVNVL STVDALVLTE VYAAGEAAIT TANGDALSRA LRAVGRVDPV 430 440 450 460 FVASVDDVPD ALAGVARNGD VVITMGAGSI GGVPAKIVQH IQQKA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000440 Genomic DNA. Translation: ABI86023.1.
RefSeq	YP_772357.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4311153.
GenomeReviews	Gene locus Bamb_0464 in contig CP000440_GR.
KEGG	bam:Bamb_0464.
NMPDR	fig|339670.3.peg.6101.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q0BIK0. EWMVVEA.
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BURCM

Accession

Primary (citable) accession number: Q0BIK0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)