Reviewed, UniProtKB/Swiss-Prot Q0BI48 (KATG1_BURCM)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Ordered Locus Names:	Bamb_0616

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354730
Sites
	Active site	92	1	Proton acceptor By similarity
	Metal binding	259	1	Iron (heme axial ligand) By similarity
	Site	88	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	91 ↔ 218		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-244) By similarity
	Cross-link	218 ↔ 244		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-91) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BI48-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 625EF0DD9823320A	FASTA	728	79,133
	10 20 30 40 50 60 MSTEAKCPFN HTAAGGTSNK DWWPNQLNLN ILHRHSALSD PMDKDFDYAQ AFKKLDLAAV 70 80 90 100 110 120 KRDLQALMTT SQDWWPADFG HYGGLFVRMA WHSAGTYRIA DGRGGAGGGQ QRFAPLNSWP 130 140 150 160 170 180 DNANLDKARR LLWPIKQKYG RNISWADLLI LTGNVALESM GFKTFGYAGG RADTWEPDDV 190 200 210 220 230 240 YWGSEKIWLE LSGGPNSRYS GDRDLENPLA AVQMGLIYVN PEGPDGNPDP VAAARDIRDT 250 260 270 280 290 300 FARMAMNDEE TVALIAGGHT FGKTHGAGPA TDVGPEPEAA GIELQGLGWK SAYASGKGHD 310 320 330 340 350 360 AITSGLEVTW TSTPTKWSHD FFKHLFSYEW ELTKSPAGAH QWVAKGADEV IPDAFDASKK 370 380 390 400 410 420 HRPTMLTTDL SLRFDPAYEK ISRRFYENPA EFADAFARAW FKLTHRDMGP RARYLGPEVP 430 440 450 460 470 480 AEELLWQDPI PAVDHPLIDD ADVAALKAKV LASGLSVAQL VSTAWASAST FRGSDKRGGA 490 500 510 520 530 540 NGARIRLAPQ KDWEVNQPAA LAAVLETLEG VRKAFNDAQT GGKKVSLADL IVLAGAAGVE 550 560 570 580 590 600 QAAKSAGVAV TVPFAPGRMD ASQEQTDVDA MAVLEPVADG FRNYLKSAYK TPAEALLVDK 610 620 630 640 650 660 AQLLTLNGPE LTVLVGGLRV LGANVGDAKH GVFTDRPGTL SNDFFVNLLD MGTEWKPVSA 670 680 690 700 710 720 ANDVFEGRDR ATGQVKWTGT RVDLIFGSHS QLRALAEVYG SADANEKFVR DFVAAWNKVM NLDRFDLA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000440 Genomic DNA. Translation: ABI86175.1.
RefSeq	YP_772509.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4311557.
GenomeReviews	Gene locus Bamb_0616 in contig CP000440_GR.
KEGG	bam:Bamb_0616.
NMPDR	fig|339670.3.peg.3192.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q0BI48. FEWELTK.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_BURCM

Accession

Primary (citable) accession number: Q0BI48

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)