Reviewed, UniProtKB/Swiss-Prot Q0BI24 (HEMH_BURCM)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	Bamb_0640

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Ferrochelatase HAMAP MF_00323		PRO_1000019278
Sites
	Metal binding	214	1	Iron By similarity
	Metal binding	295	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BI24-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 5A3846DB1970472F	FASTA	354	39,156
	10 20 30 40 50 60 MRFDLEPPSS VAAAHRTGVL LINLGTPDAP TPRAVRRYLA EFLSDPRVVE IPQVVWQVLL 70 80 90 100 110 120 RTLILPLRGR ASAKKYAAVW MPEGSPLRVY TERQTEGVRH LLASNAYQVT VDYAMRYGSP 130 140 150 160 170 180 NIAQALAQFK RAGVERVLLM PMYPQYSAST TATAFDAAFA ALARMRNQPE VRTVRQYADH 190 200 210 220 230 240 PAYIHALAEQ VRQYWAQHGR PDFAAGDKLV LSFHGVPKRT LDLGDPYHDQ CQQTGALLMA 250 260 270 280 290 300 ALGLSTLECR VTFQSRFGKA EWLQPYTAPT LRELGAAGVR RADVFCPGFT ADCLETIEEI 310 320 330 340 350 GMEVRDEFIA GGGQAFHRIP CLNGAHAWIG ALGEIVAENL QGWPVKAAQP DMVN

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000440 Genomic DNA. Translation: ABI86199.1.
RefSeq	YP_772533.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BI24.
Genome annotation databases
GeneID	4309287.
GenomeReviews	Gene locus Bamb_0640 in contig CP000440_GR.
KEGG	bam:Bamb_0640.
NMPDR	fig|339670.3.peg.3167.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0276.
HOGENOM	HBG697135.
OMA	TIEEIGM.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_BURCM

Accession

Primary (citable) accession number: Q0BI24

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	February 9, 2010
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)