Reviewed, UniProtKB/Swiss-Prot Q0BI15 (PANB1_BURCM)

Names and origin

Protein names

Recommended name:
    3-methyl-2-oxobutanoate hydroxymethyltransferase 1
    EC=2.1.2.11
Alternative name(s):
    Ketopantoate hydroxymethyltransferase 1
      Short name=KPHMT 1

Gene names

Name:	panB1
Ordered Locus Names:	Bamb_0649

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the panB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	3-methyl-2-oxobutanoate hydroxymethyltransferase 1 HAMAP MF_00156		PRO_0000297231
Regions
	Region	53 – 54	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	189	1	Proton acceptor By similarity
	Metal binding	53	1	Magnesium By similarity
	Metal binding	92	1	Magnesium By similarity
	Metal binding	122	1	Magnesium By similarity
	Binding site	92	1	Alpha-ketoisovalerate By similarity
	Binding site	120	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BI15-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 1C650F489D4B7D26	FASTA	271	28,740
	10 20 30 40 50 60 MTYLQESSRP AVTVPKLQAM RDAGEKIAML TCYDASFAAL LDRSGVDVLL IGDSLGNVLQ 70 80 90 100 110 120 GHTTTLPVSL DDIAYHTACV ARAQPRALII ADLPFGTYGT PAEAFASAVK LMRAGAQMIK 130 140 150 160 170 180 LEGGEWLAET IRFLVERAVP VCAHVGLTPQ SVHAFGGFKV QGKTEAGAAQ LLRDARAVED 190 200 210 220 230 240 AGAQLVVLEA VPTLVASEVT HMLKIPTIGI GAGTDCSGQV LVLHDMLGIF PGKRPRFVKD 250 260 270 FMQGQPTIQA AVEAYVRAVK DSSFPGPEHS F

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000440 Genomic DNA. Translation: ABI86208.1.
RefSeq	YP_772542.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BI15.
Genome annotation databases
GeneID	4309296.
GenomeReviews	Gene locus Bamb_0649 in contig CP000440_GR.
KEGG	bam:Bamb_0649.
NMPDR	fig|339670.3.peg.3159.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MSYLLDP.
Family and domain databases
HAMAP	MF_00156. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
TIGRFAMs	TIGR00222. panB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB1_BURCM

Accession

Primary (citable) accession number: Q0BI15

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)