ID   MIAA_BURCM              Reviewed;         324 AA.
AC   Q0BI05;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=tRNA Delta(2)-isopentenylpyrophosphate transferase;
DE            Short=IPP transferase;
DE            EC=2.5.1.8;
DE   AltName: Full=Isopentenyl-diphosphate:tRNA isopentenyltransferase;
DE            Short=IPTase;
DE            Short=IPPT;
DE   AltName: Full=Dimethylallyl diphosphate:tRNA dimethylallyltransferase;
DE            Short=DMAPP:tRNA dimethylallyltransferase;
DE            Short=DMATase;
GN   Name=miaA; OrderedLocusNames=Bamb_0659;
OS   Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia
OS   cepacia (strain AMMD)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=339670;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F.,
RA   Land M., Hauser L., Kyrpides N., Kim E., Parke J., Coenye T.,
RA   Konstantinidis K., Ramette A., Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome 1 of Burkholderia cepacia AMMD.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the transfer of a dimethylallyl group onto the
CC       adenine at position 37 in tRNAs that read codons beginning with
CC       uridine, leading to the formation of N6-(dimethylallyl)adenosine
CC       (i(6)A) (By similarity).
CC   -!- CATALYTIC ACTIVITY: Isopentenyl diphosphate + tRNA = diphosphate +
CC       tRNA containing 6-isopentenyladenosine.
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- SUBUNIT: Monomer (By similarity).
CC   -!- SIMILARITY: Belongs to the IPP transferase family.
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DR   EMBL; CP000440; ABI86218.1; -; Genomic_DNA.
DR   RefSeq; YP_772552.1; -.
DR   GeneID; 4311312; -.
DR   GenomeReviews; CP000440_GR; Bamb_0659.
DR   KEGG; bam:Bamb_0659; -.
DR   NMPDR; fig|339670.3.peg.3149; -.
DR   OMA; Q0BI05; VNADSMQ.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0004811; F:tRNA isopentenyltransferase activity; IEA:HAMAP.
DR   GO; GO:0008033; P:tRNA processing; IEA:HAMAP.
DR   HAMAP; MF_00185; -; 1.
DR   InterPro; IPR002627; IPPT.
DR   InterPro; IPR018022; tRNA_delta_PyrP_Trfase.
DR   PANTHER; PTHR11088; IPPT; 1.
DR   Pfam; PF01715; IPPT; 1.
DR   ProDom; PD004674; IPPT; 1.
DR   ProDom; PD005388; IPPtrans_like; 1.
DR   TIGRFAMs; TIGR00174; miaA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Magnesium; Nucleotide-binding;
KW   Nucleotidyltransferase; Transferase; tRNA processing.
FT   CHAIN         1    324       tRNA Delta(2)-isopentenylpyrophosphate
FT                                transferase.
FT                                /FTId=PRO_1000020572.
FT   NP_BIND      17     24       ATP (Potential).
FT   REGION       19     24       Substrate binding (By similarity).
FT   REGION       42     45       Interaction with substrate tRNA (By
FT                                similarity).
FT   REGION      166    170       Interaction with substrate tRNA (By
FT                                similarity).
FT   REGION      251    256       Interaction with substrate tRNA (By
FT                                similarity).
FT   REGION      284    291       Interaction with substrate tRNA (By
FT                                similarity).
FT   SITE        108    108       Interaction with substrate tRNA (By
FT                                similarity).
FT   SITE        130    130       Interaction with substrate tRNA (By
FT                                similarity).
SQ   SEQUENCE   324 AA;  35584 MW;  2654BB1163F3E3C5 CRC64;
     MSASPQSRPT TITCLLGPTA SGKTAAALAL AARRPIEIVS VDSALVYRDM DIGTAKPTRD
     ERARVPHHLI DIIDPADAYS AAEFRADALR LIGEIAARGR TPLLAGGTML YYKALTQGLN
     DLPTADPAVR AELDADAARD GWPALHARLA QVDPATAARL APNDSQRIQR ALEVFLLSGQ
     PMSVLLAAPR RADDEAAAYR FVPVALEPSD RAVLHARIAQ RFDAMLDAGF IDEVERLRRR
     EDLHLGLPSM RCVGYRQAWE FLDGDTDYRT MRDKGIFATR QLCKRQITWL RAMPERIVVD
     CVAPDATALA LDTLERVLDD RLPT
//