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Q0BI05 (MIAA_BURCM) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA dimethylallyltransferase
EC=2.5.1.75
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferase
Short name=DMAPP:tRNA dimethylallyltransferase
Short name=DMATase
Isopentenyl-diphosphate:tRNA isopentenyltransferase
Short name=IPP transferase
Short name=IPPT
Short name=IPTase
Gene names
Name:miaA
Ordered Locus Names:Bamb_0659
OrganismBurkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]
Taxonomic identifier339670 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A) By similarity. HAMAP MF_00185

Catalytic activity

Dimethylallyl diphosphate + tRNA = diphosphate + tRNA containing 6-dimethylallyladenosine. HAMAP MF_00185

Cofactor

Magnesium By similarity. HAMAP MF_00185

Subunit structure

Monomer By similarity. HAMAP MF_00185

Sequence similarities

Belongs to the IPP transferase family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandATP-binding
Magnesium
Nucleotide-binding
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

tRNA dimethylallyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324tRNA dimethylallyltransferase HAMAP MF_00185
PRO_1000020572

Regions

Nucleotide binding17 – 248ATP Potential
Region19 – 246Substrate binding By similarity
Region42 – 454Interaction with substrate tRNA By similarity
Region166 – 1705Interaction with substrate tRNA By similarity
Region251 – 2566Interaction with substrate tRNA By similarity
Region284 – 2918Interaction with substrate tRNA By similarity

Sites

Site1081Interaction with substrate tRNA By similarity
Site1301Interaction with substrate tRNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0BI05 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 2654BB1163F3E3C5

FASTA32435,584
        10         20         30         40         50         60 
MSASPQSRPT TITCLLGPTA SGKTAAALAL AARRPIEIVS VDSALVYRDM DIGTAKPTRD 

        70         80         90        100        110        120 
ERARVPHHLI DIIDPADAYS AAEFRADALR LIGEIAARGR TPLLAGGTML YYKALTQGLN 

       130        140        150        160        170        180 
DLPTADPAVR AELDADAARD GWPALHARLA QVDPATAARL APNDSQRIQR ALEVFLLSGQ 

       190        200        210        220        230        240 
PMSVLLAAPR RADDEAAAYR FVPVALEPSD RAVLHARIAQ RFDAMLDAGF IDEVERLRRR 

       250        260        270        280        290        300 
EDLHLGLPSM RCVGYRQAWE FLDGDTDYRT MRDKGIFATR QLCKRQITWL RAMPERIVVD 

       310        320 
CVAPDATALA LDTLERVLDD RLPT

« Hide

References

[1]"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-244 / AMMD.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000440 Genomic DNA. Translation: ABI86218.1.
RefSeqYP_772552.1. NC_008390.1.

3D structure databases

ProteinModelPortalQ0BI05.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0BI05.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4311312.
GenomeReviewsGene locus Bamb_0659 in contig CP000440_GR.
KEGGbam:Bamb_0659.
NMPDRfig|339670.3.peg.3149.
PATRIC19016830. VBIBurAmb61564_0725.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0324.
HOGENOMHBG559459.
OMAHTRNYAK.
PhylomeDBQ0BI05.
ProtClustDBPRK00091.

Enzyme and pathway databases

BioCycBAMB339670:BAMB_0659-MONOMER.

Family and domain databases

HAMAPMF_00185. IPP_trans.
[Tree]
InterProIPR002627. IPPT.
IPR018022. tRNA_delta_PyrP_Trfase.
[Graphical view]
KOK00791.
PANTHERPTHR11088. IPPT. 1 hit.
PfamPF01715. IPPT. 1 hit.
[Graphical view]
TIGRFAMsTIGR00174. MiaA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMIAA_BURCM
AccessionPrimary (citable) accession number: Q0BI05
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 17, 2006
Last modified: January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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