Reviewed, UniProtKB/Swiss-Prot Q0BI05 (MIAA_BURCM)

Names and origin

Protein names

Recommended name:
    tRNA Delta(2)-isopentenylpyrophosphate transferase
      Short name=IPP transferase
    EC=2.5.1.8
Alternative name(s):
    Isopentenyl-diphosphate:tRNA isopentenyltransferase
      Short name=IPTase
      Short name=IPPT
    Dimethylallyl diphosphate:tRNA dimethylallyltransferase
      Short name=DMAPP:tRNA dimethylallyltransferase
      Short name=DMATase

Gene names

Name:	miaA
Ordered Locus Names:	Bamb_0659

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A) By similarity.
Catalytic activity	Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine. HAMAP MF_00185
Cofactor	Magnesium By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the IPP transferase family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP nucleotidyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW tRNA isopentenyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	tRNA Delta(2)-isopentenylpyrophosphate transferase		PRO_1000020572
Regions
	Nucleotide binding	17 – 24	8	ATP Potential
	Region	19 – 24	6	Substrate binding By similarity
	Region	42 – 45	4	Interaction with substrate tRNA By similarity
	Region	166 – 170	5	Interaction with substrate tRNA By similarity
	Region	251 – 256	6	Interaction with substrate tRNA By similarity
	Region	284 – 291	8	Interaction with substrate tRNA By similarity
Sites
	Site	108	1	Interaction with substrate tRNA By similarity
	Site	130	1	Interaction with substrate tRNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BI05-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 2654BB1163F3E3C5	FASTA	324	35,584
	10 20 30 40 50 60 MSASPQSRPT TITCLLGPTA SGKTAAALAL AARRPIEIVS VDSALVYRDM DIGTAKPTRD 70 80 90 100 110 120 ERARVPHHLI DIIDPADAYS AAEFRADALR LIGEIAARGR TPLLAGGTML YYKALTQGLN 130 140 150 160 170 180 DLPTADPAVR AELDADAARD GWPALHARLA QVDPATAARL APNDSQRIQR ALEVFLLSGQ 190 200 210 220 230 240 PMSVLLAAPR RADDEAAAYR FVPVALEPSD RAVLHARIAQ RFDAMLDAGF IDEVERLRRR 250 260 270 280 290 300 EDLHLGLPSM RCVGYRQAWE FLDGDTDYRT MRDKGIFATR QLCKRQITWL RAMPERIVVD 310 320 CVAPDATALA LDTLERVLDD RLPT

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000440 Genomic DNA. Translation: ABI86218.1.
RefSeq	YP_772552.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4311312.
GenomeReviews	Gene locus Bamb_0659 in contig CP000440_GR.
KEGG	bam:Bamb_0659.
NMPDR	fig|339670.3.peg.3149.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q0BI05. VNADSMQ.
Family and domain databases
HAMAP	MF_00185. [Tree]
InterPro	IPR002627. IPPT. IPR018022. tRNA_delta_PyrP_Trfase. [Graphical view]
PANTHER	PTHR11088. IPPT. 1 hit.
Pfam	PF01715. IPPT. 1 hit. [Graphical view]
ProDom	PD004674. IPPT. 1 hit. PD005388. IPPtrans_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00174. miaA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MIAA_BURCM

Accession

Primary (citable) accession number: Q0BI05

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)