Reviewed, UniProtKB/Swiss-Prot Q0BHW3 (HGD_BURCM)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	Bamb_0701

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O(2) = 4-maleylacetoacetate.
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 4/6.
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Homogentisate 1,2-dioxygenase		PRO_1000019523
Sites
	Metal binding	341	1	Iron By similarity
	Metal binding	347	1	Iron By similarity
	Metal binding	377	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BHW3-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: FA49EAFE21E728E2	FASTA	444	49,032
	10 20 30 40 50 60 MTLDLSKPAT AGYLSGFANE FATEALPGAL PHGRNSPQRA PYGLYAEQLS GTAFTAPRGH 70 80 90 100 110 120 NRRSWLYRIR PAAVHRPFEP FTGPQRLVSE FGDSADVPPT PPNQLRWDPL PMPVEPTDFV 130 140 150 160 170 180 EGLVTMAGNG SAAAMNGCAI HLYAANRSMQ DRFFYSADGE LLIVPQQGRL FIATEFGRLD 190 200 210 220 230 240 VEPFEIAVIP RGVRFSVALP DGDARGYICE NFGALLRLPD LGPIGSNGLA NPRDFLTPQA 250 260 270 280 290 300 AYEDREGAFE LVAKLNGRLW RADIGHSPFD VVAWHGNYAP YKYDLRLFNT IGSISFDHPD 310 320 330 340 350 360 PSIFLVLQSQ SDTPGVDAID FVIFPPRWLA AEDTFRPPWF HRNVASEFMG LVHGAYDAKA 370 380 390 400 410 420 EGFVPGGASL HNCMSGHGPD ADTFEKASAS DTTKPHKVDA TMAFMFETRT LIRPTRYALD 430 440 TAQLQPDYFE CWQGIRKHFN PEQP

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000440 Genomic DNA. Translation: ABI86260.1.
RefSeq	YP_772594.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4309714.
GenomeReviews	Gene locus Bamb_0701 in contig CP000440_GR.
KEGG	bam:Bamb_0701.
NMPDR	fig|339670.3.peg.3109.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00334. [Tree]
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_BURCM

Accession

Primary (citable) accession number: Q0BHW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	November 4, 2008
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)