ID   TRMB_BURCM              Reviewed;         255 AA.
AC   Q0BHL9;
DT   29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   16-JUN-2009, entry version 19.
DE   RecName: Full=tRNA (guanine-N(7)-)-methyltransferase;
DE            EC=2.1.1.33;
DE   AltName: Full=tRNA(m7G46)-methyltransferase;
GN   Name=trmB; OrderedLocusNames=Bamb_0795;
OS   Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia
OS   cepacia (strain AMMD)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=339670;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F.,
RA   Land M., Hauser L., Kyrpides N., Kim E., Parke J., Coenye T.,
RA   Konstantinidis K., Ramette A., Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome 1 of Burkholderia cepacia AMMD.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of N(7)-methylguanine at
CC       position 46 (m7G46) in tRNA (By similarity).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-
CC       homocysteine + tRNA containing N(7)-methylguanine.
CC   -!- PATHWAY: tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. TrmB
CC       family.
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DR   EMBL; CP000440; ABI86354.1; -; Genomic_DNA.
DR   RefSeq; YP_772688.1; -.
DR   GeneID; 4308978; -.
DR   GenomeReviews; CP000440_GR; Bamb_0795.
DR   KEGG; bam:Bamb_0795; -.
DR   NMPDR; fig|339670.3.peg.3019; -.
DR   OMA; Q0BHL9; TKFENRG.
DR   GO; GO:0008176; F:tRNA (guanine-N7-)-methyltransferase activity; IEA:HAMAP.
DR   GO; GO:0006400; P:tRNA modification; IEA:HAMAP.
DR   HAMAP; MF_01057; -; 1.
DR   InterPro; IPR003358; tRNA_(Gua-N-7)_MeTrfase.
DR   PANTHER; PTHR23417:SF1; Methyltransf_4; 1.
DR   Pfam; PF02390; Methyltransf_4; 1.
DR   TIGRFAMs; TIGR00091; CHP91; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase; tRNA processing.
FT   CHAIN         1    255       tRNA (guanine-N(7)-)-methyltransferase.
FT                                /FTId=PRO_0000288129.
FT   REGION      232    235       Substrate binding (Potential).
FT   BINDING      86     86       S-adenosyl-L-methionine (By similarity).
FT   BINDING     111    111       S-adenosyl-L-methionine (By similarity).
FT   BINDING     138    138       S-adenosyl-L-methionine (By similarity).
FT   BINDING     161    161       S-adenosyl-L-methionine (By similarity).
FT   BINDING     165    165       Substrate (By similarity).
FT   BINDING     197    197       Substrate (Potential).
SQ   SEQUENCE   255 AA;  28399 MW;  C98B681217D2EFE7 CRC64;
     MMHDDPNEAG LPPDDAALPD EAADGADEVN PLHHRRIRSF VTRAGRVSTG QRRALDEFGP
     RFVVPYAAEI PDWDAVFGRS APRILEIGFG MGASTAEIAA HRPGDDFLGV EVHEPGVGAL
     LKLIGEQDLP NIRIIQHDAV EVLEHMLAPE SLDGVHIFFP DPWHKARHHK RRLIQPPLVA
     HLASRLKPGA YLHCATDWQN YAEQMLEVLG AEPSLENTAA DYAPRPDYRP ITKFERRGLR
     LGHGVWDLVF RKRAG
//