Q0BH67 (TRMD_BURCM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	Bamb_0947

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000006456
Regions
	Region	145 – 150	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	125	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BH67 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 72C599F9BCDF6715	FASTA	264	29,439
	10 20 30 40 50 60 MNQVTESAVQ FDVVTLFPEM FRALTDWGIT SRAVKQERFG LRTWNPRDFT TDNYRTVDDR 70 80 90 100 110 120 PYGGGPGMVM LAKPLEAAIG AAKAAQAEQG VASTRVVMMS PQGAPFTHER AVRMAQEPGV 130 140 150 160 170 180 IVLCGRYEAI DQRLLDRCVD EEISLGDFVL SGGELPAMAM MDAVVRLLPG VLNDAQSAVQ 190 200 210 220 230 240 DSFVDGLLDC PHYTRPEEYE GMRVPDVLLG GHHAEIERWR RQEALKNTLR KRPDLIVRAR 250 260 REKLLSRADE AWLANLAREA KNAS

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-244 / AMMD.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000440 Genomic DNA. Translation: ABI86506.1.
RefSeq	YP_772840.1. NC_008390.1.
3D structure databases
ProteinModelPortal	Q0BH67.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BH67.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4308685.
GenomeReviews	Gene locus Bamb_0947 in contig CP000440_GR.
KEGG	bam:Bamb_0947.
NMPDR	fig|339670.3.peg.1975.
PATRIC	19017440. VBIBurAmb61564_1022.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0336.
HOGENOM	HBG285805.
OMA	VCGRFEG.
PhylomeDB	Q0BH67.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	BAMB339670:BAMB_0947-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BURCM

Accession

Primary (citable) accession number: Q0BH67

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program