ID   SYT_BURCM               Reviewed;         635 AA.
AC   Q0BG06;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   16-JUN-2009, entry version 23.
DE   RecName: Full=Threonyl-tRNA synthetase;
DE            EC=6.1.1.3;
DE   AltName: Full=Threonine--tRNA ligase;
DE            Short=ThrRS;
GN   Name=thrS; OrderedLocusNames=Bamb_1359;
OS   Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia
OS   cepacia (strain AMMD)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=339670;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F.,
RA   Land M., Hauser L., Kyrpides N., Kim E., Parke J., Coenye T.,
RA   Konstantinidis K., Ramette A., Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome 1 of Burkholderia cepacia AMMD.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: ATP + L-threonine + tRNA(Thr) = AMP +
CC       diphosphate + L-threonyl-tRNA(Thr).
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase
CC       family.
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DR   EMBL; CP000440; ABI86917.1; -; Genomic_DNA.
DR   RefSeq; YP_773251.1; -.
DR   GeneID; 4311276; -.
DR   GenomeReviews; CP000440_GR; Bamb_1359.
DR   KEGG; bam:Bamb_1359; -.
DR   NMPDR; fig|339670.3.peg.2188; -.
DR   OMA; Q0BG06; ELELFMF.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR   GO; GO:0004829; F:threonine-tRNA ligase activity; IEA:HAMAP.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006435; P:threonyl-tRNA aminoacylation; IEA:HAMAP.
DR   HAMAP; MF_00184; -; 1.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb_cons-reg.
DR   InterPro; IPR006195; aa-tRNA-synth_II_cons-reg.
DR   InterPro; IPR004154; Anticodon_bd.
DR   InterPro; IPR012675; b-grasp_ferredoxin-like.
DR   InterPro; IPR004095; TGS.
DR   InterPro; IPR002320; Thr-tRNA-synth_IIa.
DR   InterPro; IPR018158; Thr-tRNA-synth_IIa_cons-reg.
DR   InterPro; IPR012947; tRNA_SAD.
DR   Gene3D; G3DSA:3.40.50.800; Anticodon_bd; 1.
DR   Gene3D; G3DSA:3.10.20.30; Ferredoxin_fold; 1.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF02824; TGS; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   Pfam; PF07973; tRNA_SAD; 1.
DR   PRINTS; PR01047; TRNASYNTHTHR.
DR   TIGRFAMs; TIGR00418; thrS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW   Ligase; Metal-binding; Nucleotide-binding; Protein biosynthesis; Zinc.
FT   CHAIN         1    635       Threonyl-tRNA synthetase.
FT                                /FTId=PRO_1000020351.
FT   REGION      242    533       Catalytic.
FT   METAL       333    333       Zinc; catalytic (By similarity).
FT   METAL       384    384       Zinc; catalytic (By similarity).
FT   METAL       510    510       Zinc; catalytic (By similarity).
SQ   SEQUENCE   635 AA;  72306 MW;  7C27EA357756C0EA CRC64;
     MVSIRLPDGS VRQYEHPVTV AEVAASIGPG LAKAALGGKL DGELVDTSTV IDRDAALAII
     TDKDADGLDI IRHSTAHLLA YAVKELYPDA QVTIGPVIDN GFYYDFSYNR PFTPEDLEKI
     EKRMQEIAKK DEPVTRRVVS RDEAAGYFRS IGEKYKAEII ESIPQTDEIK LYSHGGFTDL
     CRGPHVPSTG KLKVFKLMKV AGAYWRGDSK NEQLQRIYGT AWTKKEDQDQ YLHMLEEAEK
     RDHRKLGKQL DLFHMQEESP GMVFWHPKGW ALWQQVEQYM RRRVNDAGYL EIKTPMIMDR
     SLWEASGHWQ NYRENMFTTE SEKRDYAIKP MNCPGHVQVF KHGLRSYRDL PLRYAEFGSC
     HRNEASGALH GLMRVRGFVQ DDAHIFCTEE QFIAESIAFN TLAMSVYKDF GFEHIDIKLS
     LRPEQRAGTD ETWDRAEQGL RDALTACGLS WEELPGEGAF YGPKIEYHIK DALGRSWQCG
     TLQLDMVLPE RLGAEYVAED NSRRRPVMLH RAIVGSMERF LGILIEHHAG AMPAWLAPFQ
     AVVLNIAESQ VEYAHSLTQT LQKQGVRVAG DLRNEKISYK IREHTLEKVP YLLVVGDKER
     DAQTVAVRAR GGVDLGVMPI EAFVERLQED LRSFK
//