Q0BED6 (ISPD_BURCM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	Bamb_1931

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_1000022903
Sites
	Site	17	1	Transition state stabilizer By similarity
	Site	24	1	Transition state stabilizer By similarity
	Site	159	1	Positions MEP for the nucleophilic attack By similarity
	Site	215	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BED6 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: D41BCEB334E0EF36	FASTA	236	25,290
	10 20 30 40 50 60 MTPRLFALIP CAGTGSRSGS AVPKQYRTLA GRALLHYTLA AFDACSEFAQ TLVVLAPDDT 70 80 90 100 110 120 HFDARRFAGL RFAVRRCGGG SRQASVLNGL LGLGEFGATD QDWVLVHDAA RPGITPELIR 130 140 150 160 170 180 TLVAALKDDP VGGIVALPVA DTLKRVPAGG DAIARTESRD ALWQAQTPQM FRIGMLRDAI 190 200 210 220 230 LRAQREGHDL TDEASAIEWA GHTPRVVQGS LRNFKVTYPE DFALAEAILA RGTNAS

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-244 / AMMD.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000440 Genomic DNA. Translation: ABI87487.1.
RefSeq	YP_773821.1. NC_008390.1.
3D structure databases
ProteinModelPortal	Q0BED6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BED6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4310935.
GenomeReviews	Gene locus Bamb_1931 in contig CP000440_GR.
KEGG	bam:Bamb_1931.
NMPDR	fig|339670.3.peg.6154.
PATRIC	19019490. VBIBurAmb61564_2029.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	FPQGAIL.
PhylomeDB	Q0BED6.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	BAMB339670:BAMB_1931-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_BURCM

Accession

Primary (citable) accession number: Q0BED6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program