Q0BE25 (LPXD_BURCM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	Bamb_2042

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_1000050930
Sites
	Active site	258	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BE25 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: E857EE9D0FE1BB08	FASTA	364	37,203
	10 20 30 40 50 60 MALTLEELVK RFGGEIAGDA QCKVSGLAPL DQAGPQQLAF LANPKYLSQV ETTRAGAVLI 70 80 90 100 110 120 APKDLEKLRA ATGGQAAGPL NFIVTPNPYA YFARVAQMFI DLATPPRAPG VHPSATIDPA 130 140 150 160 170 180 AQVAASAVIG PHVTVEAGAV IEDGVQLDAN VFIGRGTTIG AGSHLYPNAS VYHGCKIGPR 190 200 210 220 230 240 AIIHAGAVIG SDGFGFAPDF VGDGDARTGS WVKIPQVGGV TIGPDVEIGA NTTIDRGAMA 250 260 270 280 290 300 DTVIDECVKI DNQVQIGHNC RIGAYTVIAG SAGIAGSTTI GRHCMIGGAA GIAGHVTLGD 310 320 330 340 350 360 YVIITAKSGV SKSLPKAGIY TSAFPAVDHG EWNRSAALVR NLDKLRERIK ALETALAAQG GTDA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-244 / AMMD.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000440 Genomic DNA. Translation: ABI87598.1.
RefSeq	YP_773932.1. NC_008390.1.
3D structure databases
ProteinModelPortal	Q0BE25.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BE25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4311615.
GenomeReviews	Gene locus Bamb_2042 in contig CP000440_GR.
KEGG	bam:Bamb_2042.
NMPDR	fig|339670.3.peg.6028.
PATRIC	19019696. VBIBurAmb61564_2132.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	CFVGKNT.
PhylomeDB	Q0BE25.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	BAMB339670:BAMB_2042-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 6 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_BURCM

Accession

Primary (citable) accession number: Q0BE25

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program