ID   HUTH_BURCM              Reviewed;         507 AA.
AC   Q0BDK6;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   16-JUN-2009, entry version 22.
DE   RecName: Full=Histidine ammonia-lyase;
DE            Short=Histidase;
DE            EC=4.3.1.3;
GN   Name=hutH; OrderedLocusNames=Bamb_2211;
OS   Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia
OS   cepacia (strain AMMD)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=339670;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F.,
RA   Land M., Hauser L., Kyrpides N., Kim E., Parke J., Coenye T.,
RA   Konstantinidis K., Ramette A., Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome 1 of Burkholderia cepacia AMMD.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: L-histidine = urocanate + NH(3).
CC   -!- PATHWAY: Amino-acid degradation; L-histidine degradation into L-
CC       glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Potential).
CC   -!- PTM: Contains an active site 4-methylidene-imidazol-5-one (MIO),
CC       which is formed autocatalytically by cyclization and dehydration
CC       of residues Ala-Ser-Gly (By similarity).
CC   -!- SIMILARITY: Belongs to the PAL/histidase family.
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DR   EMBL; CP000440; ABI87767.1; -; Genomic_DNA.
DR   RefSeq; YP_774101.1; -.
DR   GeneID; 4310117; -.
DR   GenomeReviews; CP000440_GR; Bamb_2211.
DR   KEGG; bam:Bamb_2211; -.
DR   NMPDR; fig|339670.3.peg.3796; -.
DR   OMA; Q0BDK6; FAPDIEA.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016211; F:ammonia ligase activity; IEA:InterPro.
DR   GO; GO:0004397; F:histidine ammonia-lyase activity; IEA:HAMAP.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   GO; GO:0006548; P:histidine catabolic process; IEA:HAMAP.
DR   HAMAP; MF_00229; -; 1.
DR   InterPro; IPR005921; HutH.
DR   InterPro; IPR001106; Phe/His_NH3-lyase.
DR   Pfam; PF00221; PAL; 1.
DR   TIGRFAMs; TIGR01225; hutH; 1.
DR   PROSITE; PS00488; PAL_HISTIDASE; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Histidine metabolism; Lyase.
FT   CHAIN         1    507       Histidine ammonia-lyase.
FT                                /FTId=PRO_1000021549.
FT   MOD_RES     142    142       2,3-didehydroalanine (Ser) (By
FT                                similarity).
FT   CROSSLNK    141    143       5-imidazolinone (Ala-Gly) (By
FT                                similarity).
SQ   SEQUENCE   507 AA;  53309 MW;  77E97E1B72B5549B CRC64;
     MITLTPGHLT LPQLRKIARE SVQLTLDPAS FAKIDAGAKA VADIAAKGEP AYGINTGFGR
     LASTHIPHDQ LELLQKNLVL SHAVGVGEPM ARSSVRLLMA LKLSSLGRGH SGIRREVMDA
     LITLFNADVL PLIPVKGSVG ASGDLAPLAH MSAVLLGVGE VFIRGERASA LDGLRVAGLA
     PLTLQAKEGL ALLNGTQAST ALALDNMFSI EDLYRTALVA GALSVDAAAG SVKPFDARIH
     ELRGHRGQID AAAAYRDLLD GSPINQSHRD CDKVQDPYSL RCQPQVMGAC LDQMRHAADV
     LLIEANAVSD NPLIFPDTGE VLSGGNFHAE PVAFAADNLA LAAAEIGALA ERRIALLIDA
     TLSGLPPFLV KDGGVNSGFM IAHVTAAALA SENKTLAHPA SVDSLPTSAN QEDHVSMATF
     AARKLADIAD NTKYILAIEL LAAAQGVDLR APYHTSPKLA PVMETIRGHV AHYELDHYFA
     PDIAVIAKLV SERAFAKVAP FSFASEQ
//