Reviewed, UniProtKB/Swiss-Prot Q0BDK6 (HUTH_BURCM)

Names and origin

Protein names

Recommended name:
    Histidine ammonia-lyase
      Short name=Histidase
    EC=4.3.1.3

Gene names

Name:	hutH
Ordered Locus Names:	Bamb_2211

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-histidine = urocanate + NH3. HAMAP MF_00229
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 1/3. HAMAP MF_00229
Subcellular location	Cytoplasm Potential.
Post-translational modification	Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly By similarity.
Sequence similarities	Belongs to the PAL/histidase family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro histidine catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ammonia ligase activity Inferred from electronic annotation. Source: InterPro histidine ammonia-lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	Histidine ammonia-lyase HAMAP MF_00229		PRO_1000021549
Amino acid modifications
	Modified residue	142	1	2,3-didehydroalanine (Ser) By similarity
	Cross-link	141 ↔ 143		5-imidazolinone (Ala-Gly) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BDK6-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 77E97E1B72B5549B	FASTA	507	53,309
	10 20 30 40 50 60 MITLTPGHLT LPQLRKIARE SVQLTLDPAS FAKIDAGAKA VADIAAKGEP AYGINTGFGR 70 80 90 100 110 120 LASTHIPHDQ LELLQKNLVL SHAVGVGEPM ARSSVRLLMA LKLSSLGRGH SGIRREVMDA 130 140 150 160 170 180 LITLFNADVL PLIPVKGSVG ASGDLAPLAH MSAVLLGVGE VFIRGERASA LDGLRVAGLA 190 200 210 220 230 240 PLTLQAKEGL ALLNGTQAST ALALDNMFSI EDLYRTALVA GALSVDAAAG SVKPFDARIH 250 260 270 280 290 300 ELRGHRGQID AAAAYRDLLD GSPINQSHRD CDKVQDPYSL RCQPQVMGAC LDQMRHAADV 310 320 330 340 350 360 LLIEANAVSD NPLIFPDTGE VLSGGNFHAE PVAFAADNLA LAAAEIGALA ERRIALLIDA 370 380 390 400 410 420 TLSGLPPFLV KDGGVNSGFM IAHVTAAALA SENKTLAHPA SVDSLPTSAN QEDHVSMATF 430 440 450 460 470 480 AARKLADIAD NTKYILAIEL LAAAQGVDLR APYHTSPKLA PVMETIRGHV AHYELDHYFA 490 500 PDIAVIAKLV SERAFAKVAP FSFASEQ

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000440 Genomic DNA. Translation: ABI87767.1.
RefSeq	YP_774101.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4310117.
GenomeReviews	Gene locus Bamb_2211 in contig CP000440_GR.
KEGG	bam:Bamb_2211.
NMPDR	fig|339670.3.peg.3796.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q0BDK6. FAPDIEA.
Family and domain databases
HAMAP	MF_00229. [Tree]
InterPro	IPR005921. HutH. IPR001106. Phe/His_NH3-lyase. [Graphical view]
Pfam	PF00221. PAL. 1 hit. [Graphical view]
TIGRFAMs	TIGR01225. hutH. 1 hit.
PROSITE	PS00488. PAL_HISTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HUTH_BURCM

Accession

Primary (citable) accession number: Q0BDK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)