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Q0BDC6 (PNP_BURCM) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Polyribonucleotide nucleotidyltransferase
EC=2.7.7.8
Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase
Gene names
Name:pnp
Ordered Locus Names:Bamb_2291
OrganismBurkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]
Taxonomic identifier339670 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length714 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP MF_01595

Catalytic activity

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595

Subunit structure

Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.

Subcellular location

Cytoplasm By similarity HAMAP MF_01595.

Sequence similarities

Belongs to the polyribonucleotide nucleotidyltransferase family.

Contains 1 KH domain.

Contains 1 S1 motif domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandRNA-binding
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processRNA processing

Inferred from electronic annotation. Source: InterPro

mRNA catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function3'-5'-exoribonuclease activity

Inferred from electronic annotation. Source: InterPro

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

polyribonucleotide nucleotidyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 714714Polyribonucleotide nucleotidyltransferase HAMAP MF_01595
PRO_0000329553

Regions

Domain560 – 61960KH
Domain629 – 69769S1 motif

Sequences

Sequence LengthMass (Da)Tools
Q0BDC6 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 113B5BE21B149D57

FASTA71477,008
        10         20         30         40         50         60 
MSMFNKVVKE FQWGQHKVRL ETGEVARQAS GAVIVDVEDT VVLATVVGAK SAKPGQDFFP 

        70         80         90        100        110        120 
LTVDYLEKTY SAGKIPGGFF RREGRPSEHE TLTSRLIDRP LRPLFPEGFY NEVQVVIHVL 

       130        140        150        160        170        180 
SVNPEIPADI PALIGASAAL AVSGLPFNGP VGAARVAYID NAYVLNPTRD QLKASSLDLV 

       190        200        210        220        230        240 
VAGTERAVLM VESEADQLPE DVMLGAVVFG HEQMQTAIDA IHELVREGGK PEWDWQPAPK 

       250        260        270        280        290        300 
NEALIARVTE LAHGDLLAAY QLRDKQARST KLKEVYAATS AKLEEDAQAA GTVAADKATV 

       310        320        330        340        350        360 
GNVLFDIEAK IVRSQILNGE PRIDGRDTRT VRPIEIRTGV LPRTHGSALF TRGETQALVV 

       370        380        390        400        410        420 
ATLGTKGDEQ IIDALEGEYR ERFMLHYNMP PFATGETGRV GSPKRREIGH GRLAKRALVK 

       430        440        450        460        470        480 
CLPSADEFGY SIRVVSEITE SNGSSSMASV CGGCLALMDA GVPMKAHVAG IAMGLILEGN 

       490        500        510        520        530        540 
KFAVLTDILG DEDHLGDMDF KVAGTEQGVT ALQMDIKIQG ITKEIMQVAL AQAKEGRMHI 

       550        560        570        580        590        600 
LGKMTSAVSG ANTQLSEFAP RMITIKINPE KIRDVIGKGG SVIRALTEET GTTIDISDDG 

       610        620        630        640        650        660 
VVTIASTSSE GMAEAKKRIE NITVEIEVGQ VYEGTVLKLL DFGAIVNLLP GKDGLLHISE 

       670        680        690        700        710 
IVNERVKDIN DYLKEGQQVK VKVIQTDEKG RVRLSAKALL NEAAASQSDT PPQQ

« Hide

References

[1]"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-244 / AMMD.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000440 Genomic DNA. Translation: ABI87847.1.
RefSeqYP_774181.1. NC_008390.1.

3D structure databases

ProteinModelPortalQ0BDC6.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ0BDC6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4309088.
GenomeReviewsGene locus Bamb_2291 in contig CP000440_GR.
KEGGbam:Bamb_2291.
NMPDRfig|339670.3.peg.5589.
PATRIC19020256. VBIBurAmb61564_2395.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1185.
HOGENOMHBG382411.
OMAYGETVVL.
PhylomeDBQ0BDC6.
ProtClustDBPRK11824.

Enzyme and pathway databases

BioCycBAMB339670:BAMB_2291-MONOMER.

Family and domain databases

HAMAPMF_01595. PNPase.
[Tree]
InterProIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH.
IPR004088. KH_type_1.
IPR018111. KH_type_1_subgr.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR012162. PNPase.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
KOK00962.
PANTHERPTHR11252. PNPase. 1 hit.
PfamPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFPIRSF005499. PNPase. 1 hit.
SMARTSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMSSF46915. 3_ExoRNase. 1 hit.
SSF55666. 3_ExoRNase. 2 hits.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF54211. Ribosomal_S5_D2-typ_fold. 2 hits.
TIGRFAMsTIGR03591. Polynuc_phos. 1 hit.
PROSITEPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePNP_BURCM
AccessionPrimary (citable) accession number: Q0BDC6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 17, 2006
Last modified: January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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