Reviewed, UniProtKB/Swiss-Prot Q0BD83 (HEM6_BURCM)

Names and origin

Protein names

Recommended name:
    Coproporphyrinogen-III oxidase, aerobic
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.3.3

Gene names

Name:	hemF
Ordered Locus Names:	Bamb_2334

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333
Catalytic activity	Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333
Subunit structure	Homodimer By similarity. HAMAP MF_00333
Subcellular location	Cytoplasm By similarity HAMAP MF_00333.
Sequence similarities	Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW porphyrin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	coproporphyrinogen oxidase activity Inferred from electronic annotation. Source: HAMAP protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 306	306	Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333		PRO_1000019457
Regions
	Region	55 – 64	10	Important for dimerization By similarity
	Region	115 – 117	3	Substrate binding By similarity
	Region	247 – 282	36	Important for dimerization By similarity
	Region	265 – 270	6	Substrate binding By similarity
Sites
	Active site	113	1	Proton donor By similarity
	Binding site	99	1	Substrate By similarity
	Site	182	1	Important for dimerization By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BD83-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 2B485B3C606CF0F8	FASTA	306	34,461
	10 20 30 40 50 60 MTDSTYDVPR VRTYLQGLQA RIADALGALD GTPLATDAWQ RGPEERLRGG GCTRILEGGR 70 80 90 100 110 120 VFERAGIGFS DVAGDALPPS ASAARPQLAG RGFEALGVSL VLHPRNPYCP TVHMNVRMLI 130 140 150 160 170 180 ATKPGEAPIF WFGGGMDLTP VYPFEDDARH FHQVCKDALD PFGAELYPRF KTWCDEYFFL 190 200 210 220 230 240 KHRNETRGIG GIFFDDFSEP GFERSFEMMQ SVGDAFLNAY LPIVERRAAL PYGERERDFQ 250 260 270 280 290 300 AYRRGRYVEF NLVFDRGTLF GLQSGGRTES ILMSMPPVAN WRYNWQPEPG SPEARLSEFL VPRDWV

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000440 Genomic DNA. Translation: ABI87890.1.
RefSeq	YP_774224.1.
3D structure databases
SMR	Q0BD83. Positions 5-306.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BD83.
Genome annotation databases
GeneID	4309131.
GenomeReviews	Gene locus Bamb_2334 in contig CP000440_GR.
KEGG	bam:Bamb_2334.
NMPDR	fig|339670.3.peg.5630.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0408.
HOGENOM	HBG631180.
OMA	HMNTRFV.
Family and domain databases
HAMAP	MF_00333. Coprogen_oxidas. [Tree]
InterPro	IPR001260. Coprogen_oxidas. IPR018375. Coproporphyrinogen-3_ox_CS. [Graphical view]
Gene3D	G3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
PANTHER	PTHR10755. Coprogen_oxidas. 1 hit.
Pfam	PF01218. Coprogen_oxidas. 1 hit. [Graphical view]
PIRSF	PIRSF000166. Coproporphyri_ox. 1 hit.
PRINTS	PR00073. COPRGNOXDASE.
PROSITE	PS01021. COPROGEN_OXIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM6_BURCM

Accession

Primary (citable) accession number: Q0BD83

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	January 19, 2010
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)