Reviewed, UniProtKB/Swiss-Prot Q0BCE0 (T23O_BURCM)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	kynA
Ordered Locus Names:	Bamb_2627

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Tryptophan 2,3-dioxygenase		PRO_0000360101
Regions
	Region	52 – 56	5	Substrate binding By similarity
	Region	77 – 81	5	Substrate binding By similarity
Sites
	Metal binding	266	1	Iron (heme axial ligand) By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	143	1	Substrate By similarity
	Binding site	150	1	Heme By similarity
	Binding site	280	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BCE0-1 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: E868A182F00E4B4A	FASTA	308	35,167
	10 20 30 40 50 60 MQPPGDNAPA GCPFSGAHAA QPAHEAPHVP GDAAGEAGWH DAQLDFSKSM SYGDYLSLNS 70 80 90 100 110 120 ILDAQHPLSP DHNEMLFIIQ HQTSELWMKL ALFELRGALD AVRGDALPPA FKMLARVSRI 130 140 150 160 170 180 LEQLVQAWNV LSTMTPSEYS AMRPYLGQSS GFQSYQYRQL EFLLGNKNVQ MLQPHAHRPD 190 200 210 220 230 240 ILEQVRATLE APSFYDEVVR LLARRGFPIA PERLERDWTQ PMRHDETVEA AWLEVYRHPQ 250 260 270 280 290 300 QHWELYEMAE ELVDLEDAFR QWRFRHVTTV ERIIGFKQGT GGTSGAPYLR KMLDVVLFPE LWHVRTTL

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000440 Genomic DNA. Translation: ABI88183.1. Different initiation.
RefSeq	YP_774517.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BCE0.
Genome annotation databases
GeneID	4311352.
GenomeReviews	Gene locus Bamb_2627 in contig CP000440_GR.
KEGG	bam:Bamb_2627.
NMPDR	fig|339670.3.peg.5309.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3483.
HOGENOM	HBG647485.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_BURCM

Accession

Primary (citable) accession number: Q0BCE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	February 9, 2010
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)