Reviewed, UniProtKB/Swiss-Prot Q0BC36 (GLPK_BURCM)

Names and origin

Protein names

Recommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Bamb_2731

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Glycerol kinase HAMAP MF_00186		PRO_1000020711
Regions
	Nucleotide binding	410 – 414	5	ATP By similarity
Sites
	Binding site	13	1	Substrate By similarity
	Binding site	17	1	ATP By similarity
	Binding site	83	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	266	1	ATP By similarity
	Binding site	309	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BC36-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: A1FAF9258A2D3D54	FASTA	500	54,649
	10 20 30 40 50 60 MQDQYILALD QGTTSSRAML FDRQGNIVSI AQKEFEQIYP QPGWVEHDPQ EIWSTQAGVA 70 80 90 100 110 120 AEAVTRTGLN GTSIAAIGIT NQRETTIVWD RETGQPVYNA IVWQDRRTAD FCDSLKKQGL 130 140 150 160 170 180 EAKVRAKTGL PIDSYFSATK IRWILDNVPG ARDKARQGKL AFGTVDSWLV WNFTKHELHV 190 200 210 220 230 240 TDVTNASRTM LFNIHTREWD SELLELLDIP RSMLPDVKAS SEIYGHTKTT VFASKIPLAG 250 260 270 280 290 300 IAGDQHAALF GQMCTTSGMV KNTYGTGCFL MMNTGDKPIE SKNNLVTTIA WQIGDDVQYA 310 320 330 340 350 360 LEGSIFIAGA VVQWLRDGVG IIKTAAEIEA LAASVPHTDG VYLVPAFAGL GAPHWNARAR 370 380 390 400 410 420 GSVFGVTRGT TSAHLARAAL DAIAYQSLDV LAAMEADSGI SIGELRVDGG ASANDLLMQF 430 440 450 460 470 480 QADLLGVDAV RPQITETTAL GAAYLAGLAI GYWKNLDEVR SQWQLDRRFA PSMPKEQVEQ 490 500 RMAGWQRAVR AAKAWADDTQ

References

[1]

"Complete sequence of chromosome 1 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000440 Genomic DNA. Translation: ABI88287.1.
RefSeq	YP_774621.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BC36.
Genome annotation databases
GeneID	4310427.
GenomeReviews	Gene locus Bamb_2731 in contig CP000440_GR.
KEGG	bam:Bamb_2731.
NMPDR	fig|339670.3.peg.434.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IAQREFT.
Family and domain databases
HAMAP	MF_00186. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BURCM

Accession

Primary (citable) accession number: Q0BC36

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)