ID   TRUA_BURCM              Reviewed;         270 AA.
AC   Q0BAC2;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   16-JUN-2009, entry version 20.
DE   RecName: Full=tRNA pseudouridine synthase A;
DE            EC=5.4.99.12;
DE   AltName: Full=tRNA-uridine isomerase I;
DE   AltName: Full=tRNA pseudouridylate synthase I;
GN   Name=truA; OrderedLocusNames=Bamb_3346;
OS   Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia
OS   cepacia (strain AMMD)).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=339670;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F.,
RA   Land M., Hauser L., Kyrpides N., Kim E., Parke J., Coenye T.,
RA   Konstantinidis K., Ramette A., Tiedje J., Richardson P.;
RT   "Complete sequence of chromosome 2 of Burkholderia cepacia AMMD.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Formation of pseudouridine at positions 38, 39 and 40 in
CC       the anticodon stem and loop of transfer RNAs (By similarity).
CC   -!- CATALYTIC ACTIVITY: tRNA uridine = tRNA pseudouridine.
CC   -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase truA
CC       family.
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DR   EMBL; CP000441; ABI88901.1; -; Genomic_DNA.
DR   RefSeq; YP_775235.1; -.
DR   GeneID; 4312348; -.
DR   GenomeReviews; CP000441_GR; Bamb_3346.
DR   KEGG; bam:Bamb_3346; -.
DR   NMPDR; fig|339670.3.peg.1041; -.
DR   OMA; Q0BAC2; RRYRYTI.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IEA:HAMAP.
DR   GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:HAMAP.
DR   HAMAP; MF_00171; -; 1.
DR   InterPro; IPR001406; tRNA_psdUrid_synth.
DR   Gene3D; G3DSA:3.30.70.580; PseudoU_synth_1; 1.
DR   Gene3D; G3DSA:3.30.70.660; PseudoU_synth_1; 1.
DR   PANTHER; PTHR11142; PseudoU_synth_1; 1.
DR   Pfam; PF01416; PseudoU_synth_1; 2.
DR   PIRSF; PIRSF001430; tRNA_psdUrid_synth; 1.
DR   TIGRFAMs; TIGR00071; hisT_truA; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Isomerase; tRNA processing.
FT   CHAIN         1    270       tRNA pseudouridine synthase A.
FT                                /FTId=PRO_1000017052.
FT   ACT_SITE     51     51       Nucleophile (By similarity).
SQ   SEQUENCE   270 AA;  29993 MW;  B9476CDC0F163C89 CRC64;
     MRIALGVQYD GAAFCGWQAQ PHGKTVQDAL ERALGEFACV PLHTTVAGRT DTGVHGLGQV
     VHFDTDLDRT DFSWVRGTNA FLPSTVSVQW AKPMPETFHA RFAAFERTYY YVLYVHPVRS
     PMLAGRAGWI HTPLDDDAMR AAAAHLIGEH DFSSFRSSEC QSKTPVKHLY QIDVRRSGHF
     IHFRFRANAF LHHMVRNLMG CLVAVGRGRY PADWLADVLA GRDRTLAAPT FMADGLYLAH
     VGYPAEFAVP PAQLGSVPWS SVWADLDLPS
//