Q0BAC2 (TRUA_BURCM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A
EC=5.4.99.12

Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I

Gene names

Name:	truA
Ordered Locus Names:	Bamb_3346

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP MF_00171
Catalytic activity	tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP MF_00171
Subunit structure	Homodimer By similarity. HAMAP MF_00171
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pseudouridine synthesis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	tRNA pseudouridine synthase A HAMAP MF_00171		PRO_1000017052
Sites
	Active site	51	1	Nucleophile By similarity
	Binding site	109	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0BAC2 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: B9476CDC0F163C89	FASTA	270	29,993
	10 20 30 40 50 60 MRIALGVQYD GAAFCGWQAQ PHGKTVQDAL ERALGEFACV PLHTTVAGRT DTGVHGLGQV 70 80 90 100 110 120 VHFDTDLDRT DFSWVRGTNA FLPSTVSVQW AKPMPETFHA RFAAFERTYY YVLYVHPVRS 130 140 150 160 170 180 PMLAGRAGWI HTPLDDDAMR AAAAHLIGEH DFSSFRSSEC QSKTPVKHLY QIDVRRSGHF 190 200 210 220 230 240 IHFRFRANAF LHHMVRNLMG CLVAVGRGRY PADWLADVLA GRDRTLAAPT FMADGLYLAH 250 260 270 VGYPAEFAVP PAQLGSVPWS SVWADLDLPS

References

[1]

"Complete sequence of chromosome 2 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-244 / AMMD.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000441 Genomic DNA. Translation: ABI88901.1.
RefSeq	YP_775235.1. NC_008391.1.
3D structure databases
ProteinModelPortal	Q0BAC2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0BAC2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4312348.
GenomeReviews	Gene locus Bamb_3346 in contig CP000441_GR.
KEGG	bam:Bamb_3346.
NMPDR	fig|339670.3.peg.1041.
PATRIC	19022487. VBIBurAmb61564_3501.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0101.
HOGENOM	HBG621020.
OMA	QANAFVH.
PhylomeDB	Q0BAC2.
ProtClustDB	PRK00021.
Enzyme and pathway databases
BioCyc	BAMB339670:BAMB_3346-MONOMER.
Family and domain databases
HAMAP	MF_00171. TruA. [Tree]
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
Gene3D	G3DSA:3.30.70.580. PseudoU_synth_1. 1 hit. G3DSA:3.30.70.660. PseudoU_synth_1. 1 hit.
KO	K06173.
PANTHER	PTHR11142. PseudoU_synth_1. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. HisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_BURCM

Accession

Primary (citable) accession number: Q0BAC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program