Q0B6M7 (METE_BURCM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	Bamb_4646

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000017231
Sites
	Metal binding	650	1	Zinc By similarity
	Metal binding	652	1	Zinc By similarity
	Metal binding	735	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0B6M7 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: A72957EDC8308360	FASTA	764	84,956
	10 20 30 40 50 60 MVTTHNLGFP RIGAQRELKF GLERYWKGES SRDALKALGA ELRARHWNAQ RDLDLAPIGD 70 80 90 100 110 120 FSFYDQVLDM SFTLGNLPKR VQGFHGDVLD NYFRVARGRS AQVVDEHGAC CGGVSAGEMT 130 140 150 160 170 180 KWFDTNYHYI VPEFHADTNF SLDPSCLLQQ LAEARALGVA GKPVILGPVT YLWLGKEKDD 190 200 210 220 230 240 SDRLALLPKL LPVYGALLDT LTAQGVEWVQ IDEPILVTEL DAEWRQALRT AYAALETRRI 250 260 270 280 290 300 KLLLATYFGT LGDNLTLAAS LPVDGLHVDA INARDEVDAL VRELPADRVL SVGAINGRNI 310 320 330 340 350 360 WKTDLNATLD WLEPLAKQLG DRLWIAPSCS LLHVPVDLAS EEKLDAEIRS WLAFALQKLD 370 380 390 400 410 420 ELKVLATALN QGRDKVADAL AANAAAIDSR RRSPRVNNPA VKAALARIDA QLGNRASPYT 430 440 450 460 470 480 QRAPKQSARL NLPAFPTTTI GSFPQTADIR HARSQFKAGA LDEAGYRAAM QAEIERSVRE 490 500 510 520 530 540 QESLGLDVLV HGEAERNDMV EYFGEQLDGY AFSQFGWVQS YGSRCVKPPI LFGDISRPKA 550 560 570 580 590 600 MTVEWITYAQ SLTNKPMKGM LTGPVTILNW SFVRDDQPRS VSCYQLALAI RDEVLDLEKA 610 620 630 640 650 660 GVRVIQIDEA ALREGLPLRR AQWGEYLKWA VEAFRITANG VQDDTQIHTH MCYSEFNDII 670 680 690 700 710 720 ASIADMDADV ITIETSRSDM ELLDAFDSFR YPNEIGPGVY DIHSPNIPTQ DHIVGLMKKA 730 740 750 760 AERIPAERLW VNPDCGLKTR QWAEVIPALT NMVAAAKTLR NQVQ

References

[1]

"Complete sequence of chromosome 2 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-244 / AMMD.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000441 Genomic DNA. Translation: ABI90196.1.
RefSeq	YP_776530.1. NC_008391.1.
3D structure databases
ProteinModelPortal	Q0B6M7.
SMR	Q0B6M7. Positions 3-762.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0B6M7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4313538.
GenomeReviews	Gene locus Bamb_4646 in contig CP000441_GR.
KEGG	bam:Bamb_4646.
NMPDR	fig|339670.3.peg.4291.
PATRIC	19025227. VBIBurAmb61564_4869.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0620.
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	Q0B6M7.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	BAMB339670:BAMB_4646-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BURCM

Accession

Primary (citable) accession number: Q0B6M7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program