Reviewed, UniProtKB/Swiss-Prot Q0B3Y4 (GLGB_BURCM)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	Bamb_5592

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	733 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 733	733	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_1000044970
Sites
	Active site	307	1	By similarity
	Active site	342	1	By similarity
	Active site	347	1	By similarity
	Active site	410	1	By similarity
	Active site	412	1	By similarity
	Active site	467	1	By similarity
	Active site	534	1	By similarity
	Active site	535	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0B3Y4-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 9A0C19F09869CEC2	FASTA	733	81,191
	10 20 30 40 50 60 MTDMLFDRTD IDALLAGRHP DPFACLGPHT DAGRVVVRAL LPGAQRVRAV TPDGDELGAL 70 80 90 100 110 120 ACVDDAGCFA GTLAHDGRYR LAIDWPDTRQ ITDDAYAFGT LLDEAALARF AAGDPAAVLD 130 140 150 160 170 180 CLGATPTRVD GVDGVRFAVW APNAQRVSVV GDFNMWDGRR HPMRLRRPWG VWELFVPGIG 190 200 210 220 230 240 AGEHYKYELR AADGHVLPHK ADPCARATEA PPRTASVVAD TAALDAFAWH DDGWLHARPP 250 260 270 280 290 300 ADQRFRMPWS IYEVHAESWQ RVPEDMDRSA NWDELAERLI PYVRGMGFTH VEFMPISEYP 310 320 330 340 350 360 FGGSWGYQPL AQFAPSARFG PVDGFARFVD RAHAAGIGVI VDWVPAHFPN DAHGLAQFDG 370 380 390 400 410 420 SALYEHADPR EGMHPDWNTC VFNLGRNEVS AFLIASALAW ARRYHVDGIR VDAVASMLYR 430 440 450 460 470 480 DYSRNEGEWV PNIHGGRENL ESVAFLRALN DTLHGTLAPP GVVTFAEEST AWPGVTASTG 490 500 510 520 530 540 DGGLGFDFKW NMGWMHDTLS YVREDPIHRR YHHDRMTFGL VYAFSERFVL PLSHDEVVHG 550 560 570 580 590 600 KGSLAVKMPG DAWQRLATLR AYFGFMWAHP GKKLLFMGSE FAQWAEFAHD ATPHWDLLDA 610 620 630 640 650 660 PAHRGVQRLV RDLNRTYAAE PALHALDCHA SGFFWLIGDD RDNSVFAFAR RDDSGRLVVA 670 680 690 700 710 720 ICNFTPVPRP GYRIGLPAPG HWRELMNTDA AVYGGTNAGN DGAVWTEDVP SHGQSWSATL 730 RLPPLATLWL SPA

References

[1]

"Complete sequence of chromosome 3 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000442 Genomic DNA. Translation: ABI91139.1.
RefSeq	YP_777473.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q0B3Y4.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	4314529.
GenomeReviews	Gene locus Bamb_5592 in contig CP000442_GR.
KEGG	bam:Bamb_5592.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HBG287139.
OMA	PKTASIV.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BURCM

Accession

Primary (citable) accession number: Q0B3Y4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 17, 2006
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)