Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q0B3Y4 (GLGB_BURCM) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:Bamb_5592
OrganismBurkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]
Taxonomic identifier339670 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Protein attributes

Sequence length733 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7337331,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000044970

Sites

Active site4121Nucleophile By similarity
Active site4671Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0B3Y4 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 9A0C19F09869CEC2

FASTA73381,191
        10         20         30         40         50         60 
MTDMLFDRTD IDALLAGRHP DPFACLGPHT DAGRVVVRAL LPGAQRVRAV TPDGDELGAL 

        70         80         90        100        110        120 
ACVDDAGCFA GTLAHDGRYR LAIDWPDTRQ ITDDAYAFGT LLDEAALARF AAGDPAAVLD 

       130        140        150        160        170        180 
CLGATPTRVD GVDGVRFAVW APNAQRVSVV GDFNMWDGRR HPMRLRRPWG VWELFVPGIG 

       190        200        210        220        230        240 
AGEHYKYELR AADGHVLPHK ADPCARATEA PPRTASVVAD TAALDAFAWH DDGWLHARPP 

       250        260        270        280        290        300 
ADQRFRMPWS IYEVHAESWQ RVPEDMDRSA NWDELAERLI PYVRGMGFTH VEFMPISEYP 

       310        320        330        340        350        360 
FGGSWGYQPL AQFAPSARFG PVDGFARFVD RAHAAGIGVI VDWVPAHFPN DAHGLAQFDG 

       370        380        390        400        410        420 
SALYEHADPR EGMHPDWNTC VFNLGRNEVS AFLIASALAW ARRYHVDGIR VDAVASMLYR 

       430        440        450        460        470        480 
DYSRNEGEWV PNIHGGRENL ESVAFLRALN DTLHGTLAPP GVVTFAEEST AWPGVTASTG 

       490        500        510        520        530        540 
DGGLGFDFKW NMGWMHDTLS YVREDPIHRR YHHDRMTFGL VYAFSERFVL PLSHDEVVHG 

       550        560        570        580        590        600 
KGSLAVKMPG DAWQRLATLR AYFGFMWAHP GKKLLFMGSE FAQWAEFAHD ATPHWDLLDA 

       610        620        630        640        650        660 
PAHRGVQRLV RDLNRTYAAE PALHALDCHA SGFFWLIGDD RDNSVFAFAR RDDSGRLVVA 

       670        680        690        700        710        720 
ICNFTPVPRP GYRIGLPAPG HWRELMNTDA AVYGGTNAGN DGAVWTEDVP SHGQSWSATL 

       730 
RLPPLATLWL SPA 

« Hide

References

[1]"Complete sequence of chromosome 3 of Burkholderia cepacia AMMD."
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P.
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-244 / AMMD.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000442 Genomic DNA. Translation: ABI91139.1.
RefSeqYP_777473.1. NC_008392.1.

3D structure databases

ProteinModelPortalQ0B3Y4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING339670.Bamb_5592.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI91139; ABI91139; Bamb_5592.
GeneID4314529.
KEGGbam:Bamb_5592.
PATRIC19027182. VBIBurAmb61564_5840.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycBAMB339670:GH48-5670-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_BURCM
AccessionPrimary (citable) accession number: Q0B3Y4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 17, 2006
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries