Reviewed, UniProtKB/Swiss-Prot Q0B385 (KATG2_BURCM)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 2
      Short name=CP 2
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 2

Gene names

Name:	katG2
Ordered Locus Names:	Bamb_5842

Organism

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) (Burkholderia cepacia (strain AMMD)) [Complete proteome] [HAMAP]

Taxonomic identifier

339670 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	741 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 741	713	Catalase-peroxidase 2 HAMAP MF_01961		PRO_5000128247
Sites
	Active site	108	1	Proton acceptor By similarity
	Metal binding	269	1	Iron (heme axial ligand) By similarity
	Site	104	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	107 ↔ 228		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-254) By similarity
	Cross-link	228 ↔ 254		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-107) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0B385-1 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: 7BDB180568080277	FASTA	741	80,910
	10 20 30 40 50 60 MQKKRVGKSV VAALAIIAMS AGTVAAWADG APRTNDFWWP ERLDLSPLRQ HDVESNPYGK 70 80 90 100 110 120 DFDYAQAFNK LDIEAVKKDI RATLTTSQDW WPADYGNYGP FFIRMAWHGA GTYRTYDGRG 130 140 150 160 170 180 GAGGAQQRFE PLNSWPDNAN LDKARRLLWP IKKKYGENIS WGDLMVLTGN VALESMGFQT 190 200 210 220 230 240 FGFGGGREDD WQSDLVYWGA GTKFMSNNRD KNGKLEKPLA ATQMGLIYVN PEGPNGNPDP 250 260 270 280 290 300 VAAAKDIRDA FGRMAMNDEE TLALIAGGHT FGKAHGAASP DKCVGAAPAG AGVEAQGLGW 310 320 330 340 350 360 ANKCGTGKGA DTITSGLEGA WSVDPVHFTM QYLDNLLEHD WVLTKSPAGA HQWMPKDAQD 370 380 390 400 410 420 IVPDAHDPSK RHPLMMFTTD IALKVDPAYS AIAKRFHAHP EEFKLAFAKA WFKLTHRDLG 430 440 450 460 470 480 PKARYLGKDV PKVDLIWQDP LPVAGYQMIG DADIAELKRR ILASGVPKAE LIKTAWASAA 490 500 510 520 530 540 SFRATDYRGG ANGARIRLAP ENDWAVNDPA SLSKVLKSLE GIQSGFNRNR TDGKQVSLAD 550 560 570 580 590 600 LIVLGGSAAV EDAARKAGYD VKVPFSPGRV DATQAQTDVA SFAVLEPTAD GFRNYYQKSN 610 620 630 640 650 660 ERSPAELMVD RASKLDLTVP EMTVLVGGLR ALDANAGHSR LGVLTNRPGT LSNDFFVNLL 670 680 690 700 710 720 DMSTQWTKSS SADGTYEGRD RKTGALKWTA SPVDLVFGSS SELRAVAEVY ASDDAHEKFV 730 740 RDFVQAWTKV MNLDRFDLKR S

References

[1]

"Complete sequence of chromosome 3 of Burkholderia cepacia AMMD." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Bruce D., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Kim E., Parke J., Coenye T., Konstantinidis K., Ramette A., Tiedje J., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000442 Genomic DNA. Translation: ABI91388.1.
RefSeq	YP_777722.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4314748.
GenomeReviews	Gene locus Bamb_5842 in contig CP000442_GR.
KEGG	bam:Bamb_5842.
NMPDR	fig|339670.3.peg.4225.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q0B385. GTDMRGG.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG2_BURCM

Accession

Primary (citable) accession number: Q0B385

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	October 17, 2006
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)