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Q0AZE0 (NADK_SYNWW) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:Swol_0584
OrganismSyntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen) [Complete proteome] [HAMAP]
Taxonomic identifier335541 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesSyntrophomonadaceaeSyntrophomonas

Protein attributes

Sequence length268 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 268268NAD kinase HAMAP-Rule MF_00361
PRO_1000059883

Regions

Nucleotide binding55 – 562NAD By similarity
Nucleotide binding129 – 1302NAD By similarity
Nucleotide binding169 – 1746NAD By similarity

Sites

Active site551Proton acceptor By similarity
Binding site601NAD By similarity
Binding site1561NAD By similarity
Binding site1581NAD By similarity
Binding site1931NAD; via carbonyl oxygen By similarity
Binding site2271NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0AZE0 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: 243D7467E040B314

FASTA26829,721
        10         20         30         40         50         60 
MKIILVNNRF KENTEKKAQE IAGQLSALNV EVIIDNGLDE PYAGTVDFIM VLGGDGTMLR 

        70         80         90        100        110        120 
AARRYGQRAI PVLGVNMGTV GFLSNIEINE LAEYLPLILR EEYSLEARMM LEVAVFQQQS 

       130        140        150        160        170        180 
LLTRVFCLNE LLLRSNSPRM LSFALEISGQ KLEPYRGDGL IVSTTTGSTA YSLSAGGPIA 

       190        200        210        220        230        240 
DPQLDAFIVT PVASHIINKR PLVVSPEREI SLHLLEEREA IIGIDGQIKM DFGAENRVLI 

       250        260 
KRAPHPLLMV NLKAKPFFTS IDRSLQRQ 

« Hide

References

[1]"The genome of Syntrophomonas wolfei: new insights into syntrophic metabolism and biohydrogen production."
Sieber J.R., Sims D.R., Han C., Kim E., Lykidis A., Lapidus A.L., McDonnald E., Rohlin L., Culley D.E., Gunsalus R., McInerney M.J.
Environ. Microbiol. 12:2289-2301(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 2245B / Goettingen.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000448 Genomic DNA. Translation: ABI67914.1.
RefSeqYP_753285.1. NC_008346.1.

3D structure databases

ProteinModelPortalQ0AZE0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING335541.Swol_0584.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI67914; ABI67914; Swol_0584.
GeneID4282681.
KEGGswo:Swol_0584.
PATRIC23855899. VBISynWol51738_0617.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227222.
KOK00858.
OMAPRKTKFV.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycSWOL335541:GHL1-603-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_SYNWW
AccessionPrimary (citable) accession number: Q0AZE0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 17, 2006
Last modified: July 9, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families