Q0AYL3 (CHEB1_SYNWW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis response regulator protein-glutamate methylesterase 1
EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	Swol_0873

Organism

Syntrophomonas wolfei subsp. wolfei (strain Goettingen) [Complete proteome] [HAMAP]

Taxonomic identifier

335541 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Syntrophomonadaceae › Syntrophomonas

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: EC two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000264325
Regions
	Domain	5 – 122	118	Response regulatory
	Domain	155 – 352	198	CheB-type methylesterase
Sites
	Active site	170	1	By similarity
	Active site	197	1	By similarity
	Active site	297	1	By similarity
Amino acid modifications
	Modified residue	56	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q0AYL3 [UniParc]. Last modified October 17, 2006. Version 1. Checksum: A58E92A5A78A5521	FASTA	352	38,352
	10 20 30 40 50 60 MPRKKVLVVD DSAFMRKMIG DMINSDDELE IVGKAGNGQE ALEKIRELQP DVVVMDIEMP 70 80 90 100 110 120 VLDGLSTLSR IMESQALPVI IFSSLSQKGT EQTLKALQLG AVDFIAKPSG QISLDVLSVK 130 140 150 160 170 180 EELIKKLKVA ANTSRKLPVY QSLHEPVVKA KKSLPDQDRK LNKLVVIAAS TGGPKALHQV 190 200 210 220 230 240 IPRFPAGIDA AILVVQHMPP GFTRSLAERL DSLSELRVKE AEHGEKVLPA CVYIAPGDYH 250 260 270 280 290 300 LKAKSRLKGT ENELYIELDQ SKPRGGLRPA ADIMLKSVAK QFWSHIVCVI MTGMGNDGAA 310 320 330 340 350 ALPCIKEKKG RIIAEHQSTC VVYGMPKAAV ETGLVDKIVP LSEITEEVLS ML

References

[1]

"Complete sequence of Syntrophomonas wolfei subsp. wolfei str. Goettingen." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Brettin T., Sims D., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Boone D.R., Brockman F., Culley D., Ferry J., Gunsalus R., McInerney M.J., Morrison M., Plugge C., Rohlin L., Scholten J., Sieber J., Stams A.J.M., Richardson P. Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Goettingen.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000448 Genomic DNA. Translation: ABI68191.1.
RefSeq	YP_753562.1. NC_008346.1.
3D structure databases
ProteinModelPortal	Q0AYL3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q0AYL3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4282004.
GenomeReviews	Gene locus Swol_0873 in contig CP000448_GR.
KEGG	swo:Swol_0873.
PATRIC	23856509. VBISynWol51738_0922.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	AKDINIS.
PhylomeDB	Q0AYL3.
ProtClustDB	CLSK958363.
Enzyme and pathway databases
BioCyc	SWOL335541:SWOL_0873-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like_superfamily. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
KO	K03412.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
SUPFAM	SSF52738. Chemotax_RR_pGlu_Me-esterase. 1 hit. SSF52172. CheY_like. 1 hit.
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_SYNWW

Accession

Primary (citable) accession number: Q0AYL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program