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Q0AYC7

- SYI_SYNWW

UniProt

Q0AYC7 - SYI_SYNWW

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Swol_0962
Organism
Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei557 – 5571Aminoacyl-adenylate By similarity
Binding sitei601 – 6011ATP By similarity
Metal bindingi895 – 8951Zinc By similarity
Metal bindingi898 – 8981Zinc By similarity
Metal bindingi915 – 9151Zinc By similarity
Metal bindingi918 – 9181Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSWOL335541:GHL1-991-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Swol_0962
OrganismiSyntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen)
Taxonomic identifieri335541 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesSyntrophomonadaceaeSyntrophomonas
ProteomesiUP000001968: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 927927Isoleucine--tRNA ligaseUniRule annotationPRO_1000022136Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi335541.Swol_0962.

Structurei

3D structure databases

ProteinModelPortaliQ0AYC7.
SMRiQ0AYC7. Positions 5-925.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotationAdd
BLAST
Motifi598 – 6025"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0AYC7-1 [UniParc]FASTAAdd to Basket

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MAKGKYDGTL NLPQTGFPMR ANLPQREPEI LKFWDEIDIY RRIQEKNAGR    50
PQFILHDGPP YANGNIHLGH TLNKVLKDII VKYRSMSGYD SPYIPGWDTH 100
GLPIEQQAIK NLGIDRHKTD VVEFREHCRD YALKYVEIQK EQFKRLGVRG 150
DWEDPYLTLS PGFESIQIKV FGEMAKKGFI YKGLKPVYWC GDCETALAEA 200
EVEYNEKVSP SIYVKFPVKD GKGVLPEDAF VIIWTTTPWT LPANTGICLH 250
PGFDYVLLEV KGEKYLLAQG LLEAVAGELG WDNYQILDKY KGEELERVIC 300
HHPFFARDSL LVLGEHVTLE AGTGCVHTAP GHGEDDFHVG QEYGLEVISP 350
VDDRGRFTAE AEKFQGLYVH DANKAVIEEL EKRNMLLKAA SIEHQYPYCW 400
RCKQPIIYRA TEQWFASIDG FRQDALNAID TVKWIPSWGR DRIFNMIRDR 450
GDWCISRQRT WGVPIPIFYC ESCGEALIND ETIQRVSELF AANGSDIWFA 500
KTAAELMPPG CSCEHCGGST FRKESDIMDV WFDSGSSHMA VLEPRQELRW 550
PSDMYLEGSD QHRGWFNSSL STAVAIRGAA PYREVLTHGF VVDEQGRKMS 600
KSLGNVVDPL RMTREMGADI LRLWVSSADY RNDVSVSPNI IKQSAEAYRK 650
IRNTCRFILG NLFDFDPGKE RVSYDKLSEL DQWALLKLDK LIRRVTKAYE 700
DYEFHVVFHS MHNFCTVDLS NIYFDILKDK LYCSHPQDAE RKAAQTVLYD 750
IINALVVMLT PILAFSSEEI WSYLKKEGQA ESVQLLEWPQ ANDEYLNQAI 800
ENRMSRVLEL REVVTKALEE ARSKKVIGHS LGAWITIYAS PEWTELLKAT 850
AGLEKIFIVS RAELKPETEA PAEALALEGV EGIRVMVQAA EGSKCERCWI 900
IENSVGEDLK HPTLCQRCAE VVAQLQG 927
Length:927
Mass (Da):105,649
Last modified:October 17, 2006 - v1
Checksum:i80F3124F415B3392
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000448 Genomic DNA. Translation: ABI68277.1.
RefSeqiWP_011640382.1. NC_008346.1.
YP_753648.1. NC_008346.1.

Genome annotation databases

EnsemblBacteriaiABI68277; ABI68277; Swol_0962.
GeneIDi4282126.
KEGGiswo:Swol_0962.
PATRICi23856699. VBISynWol51738_1014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000448 Genomic DNA. Translation: ABI68277.1 .
RefSeqi WP_011640382.1. NC_008346.1.
YP_753648.1. NC_008346.1.

3D structure databases

ProteinModelPortali Q0AYC7.
SMRi Q0AYC7. Positions 5-925.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 335541.Swol_0962.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABI68277 ; ABI68277 ; Swol_0962 .
GeneIDi 4282126.
KEGGi swo:Swol_0962.
PATRICi 23856699. VBISynWol51738_1014.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SWOL335541:GHL1-991-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of Syntrophomonas wolfei: new insights into syntrophic metabolism and biohydrogen production."
    Sieber J.R., Sims D.R., Han C., Kim E., Lykidis A., Lapidus A.L., McDonnald E., Rohlin L., Culley D.E., Gunsalus R., McInerney M.J.
    Environ. Microbiol. 12:2289-2301(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2245B / Goettingen.

Entry informationi

Entry nameiSYI_SYNWW
AccessioniPrimary (citable) accession number: Q0AYC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 17, 2006
Last modified: September 3, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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