Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei557 – 5571Aminoacyl-adenylateUniRule annotation
Binding sitei601 – 6011ATPUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSWOL335541:GHL1-991-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Swol_0962
OrganismiSyntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen)
Taxonomic identifieri335541 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesSyntrophomonadaceaeSyntrophomonas
ProteomesiUP000001968 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 927927Isoleucine--tRNA ligasePRO_1000022136Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi335541.Swol_0962.

Structurei

3D structure databases

ProteinModelPortaliQ0AYC7.
SMRiQ0AYC7. Positions 5-925.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionAdd
BLAST
Motifi598 – 6025"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q0AYC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKGKYDGTL NLPQTGFPMR ANLPQREPEI LKFWDEIDIY RRIQEKNAGR
60 70 80 90 100
PQFILHDGPP YANGNIHLGH TLNKVLKDII VKYRSMSGYD SPYIPGWDTH
110 120 130 140 150
GLPIEQQAIK NLGIDRHKTD VVEFREHCRD YALKYVEIQK EQFKRLGVRG
160 170 180 190 200
DWEDPYLTLS PGFESIQIKV FGEMAKKGFI YKGLKPVYWC GDCETALAEA
210 220 230 240 250
EVEYNEKVSP SIYVKFPVKD GKGVLPEDAF VIIWTTTPWT LPANTGICLH
260 270 280 290 300
PGFDYVLLEV KGEKYLLAQG LLEAVAGELG WDNYQILDKY KGEELERVIC
310 320 330 340 350
HHPFFARDSL LVLGEHVTLE AGTGCVHTAP GHGEDDFHVG QEYGLEVISP
360 370 380 390 400
VDDRGRFTAE AEKFQGLYVH DANKAVIEEL EKRNMLLKAA SIEHQYPYCW
410 420 430 440 450
RCKQPIIYRA TEQWFASIDG FRQDALNAID TVKWIPSWGR DRIFNMIRDR
460 470 480 490 500
GDWCISRQRT WGVPIPIFYC ESCGEALIND ETIQRVSELF AANGSDIWFA
510 520 530 540 550
KTAAELMPPG CSCEHCGGST FRKESDIMDV WFDSGSSHMA VLEPRQELRW
560 570 580 590 600
PSDMYLEGSD QHRGWFNSSL STAVAIRGAA PYREVLTHGF VVDEQGRKMS
610 620 630 640 650
KSLGNVVDPL RMTREMGADI LRLWVSSADY RNDVSVSPNI IKQSAEAYRK
660 670 680 690 700
IRNTCRFILG NLFDFDPGKE RVSYDKLSEL DQWALLKLDK LIRRVTKAYE
710 720 730 740 750
DYEFHVVFHS MHNFCTVDLS NIYFDILKDK LYCSHPQDAE RKAAQTVLYD
760 770 780 790 800
IINALVVMLT PILAFSSEEI WSYLKKEGQA ESVQLLEWPQ ANDEYLNQAI
810 820 830 840 850
ENRMSRVLEL REVVTKALEE ARSKKVIGHS LGAWITIYAS PEWTELLKAT
860 870 880 890 900
AGLEKIFIVS RAELKPETEA PAEALALEGV EGIRVMVQAA EGSKCERCWI
910 920
IENSVGEDLK HPTLCQRCAE VVAQLQG
Length:927
Mass (Da):105,649
Last modified:October 16, 2006 - v1
Checksum:i80F3124F415B3392
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000448 Genomic DNA. Translation: ABI68277.1.
RefSeqiWP_011640382.1. NC_008346.1.
YP_753648.1. NC_008346.1.

Genome annotation databases

EnsemblBacteriaiABI68277; ABI68277; Swol_0962.
KEGGiswo:Swol_0962.
PATRICi23856699. VBISynWol51738_1014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000448 Genomic DNA. Translation: ABI68277.1.
RefSeqiWP_011640382.1. NC_008346.1.
YP_753648.1. NC_008346.1.

3D structure databases

ProteinModelPortaliQ0AYC7.
SMRiQ0AYC7. Positions 5-925.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi335541.Swol_0962.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABI68277; ABI68277; Swol_0962.
KEGGiswo:Swol_0962.
PATRICi23856699. VBISynWol51738_1014.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciSWOL335541:GHL1-991-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of Syntrophomonas wolfei: new insights into syntrophic metabolism and biohydrogen production."
    Sieber J.R., Sims D.R., Han C., Kim E., Lykidis A., Lapidus A.L., McDonnald E., Rohlin L., Culley D.E., Gunsalus R., McInerney M.J.
    Environ. Microbiol. 12:2289-2301(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 2245B / Goettingen.

Entry informationi

Entry nameiSYI_SYNWW
AccessioniPrimary (citable) accession number: Q0AYC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 14, 2008
Last sequence update: October 16, 2006
Last modified: March 31, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.