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Q0AWK4 (SYL_SYNWW) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Swol_1599
OrganismSyntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen) [Complete proteome] [HAMAP]
Taxonomic identifier335541 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesSyntrophomonadaceaeSyntrophomonas

Protein attributes

Sequence length824 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 824824Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334833

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif581 – 5855"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5841ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q0AWK4 [UniParc].

Last modified October 17, 2006. Version 1.
Checksum: BDD2B77DF3ADA95E

FASTA82494,728
        10         20         30         40         50         60 
MDRKYDFKSI EAKWQAYWEN KQLFKVEEDP EKPKYYNLEM FPYPSGHLHM GHVRNYAIGD 

        70         80         90        100        110        120 
VVARFKSMNG FNVLHPMGWD AFGLPAENAA IKNAIHPATW TYSNIENMKR QLKTMGISYD 

       130        140        150        160        170        180 
WDRELATCKP DYYKFTQWMF LKLYENKLAY KKKSAVNWCP SCQTVLANEQ VVDGACERCE 

       190        200        210        220        230        240 
AVIEKKQLEQ WFFKITDYAQ RLLDDLALLP GWPEKVKTMQ KNWIGRSEGC EFSLHIEDSD 

       250        260        270        280        290        300 
EVLTVFTTRP DTVFGVTYMV LAPEHPLVEK ICHPEQEKEV KAFVNKMKYL NEMARTSTEA 

       310        320        330        340        350        360 
EKEGVFTGAY AINPMDGSRI PIWIANYVLM DYGTGAIMAV PAHDQRDFEF ARKYDIPVKV 

       370        380        390        400        410        420 
VIKGEDTPLD GNLLQESYPG DGHMVNSGNF DGLTVEEGQK AVIKFMEEKG IGQGTVNYRL 

       430        440        450        460        470        480 
RDWLISRQRY WGAPIPIVYC PECGPVAVPE EELPLYLPED IDFKPYAESP LKHLERFYKT 

       490        500        510        520        530        540 
TCPSCGKEAL RETDTMDTFV CSSWYFDRFC SPHESKQPFS REAVDYWMPV DQYIGGVEHA 

       550        560        570        580        590        600 
ILHLMYARFF TKFLFDINVL SCQEPFTRLL TQGMVLKDNA KMSKSKGNVV SPEEIIDTYG 

       610        620        630        640        650        660 
ADTARLFILF ASPPERDLEW SEQGVEGAFR FLNRVWRLVS ELAEGIKDLP AAEHFPELDS 

       670        680        690        700        710        720 
EAKNLRFKTH ATIKKVTEDI GERFNFNTAI SAIMELSNTL GAYKGIKKPN WPVVKEAVDN 

       730        740        750        760        770        780 
LLILLSPFSP HICEELWQLT GHAESIYLQK WPKYDPEALL QEEIEIVLQI SGKVRDRIMV 

       790        800        810        820 
PVDAGREELE KIALDNPKIR ELTRGKEIVK LIVVPGKLVN VVAK 

« Hide

References

[1]"The genome of Syntrophomonas wolfei: new insights into syntrophic metabolism and biohydrogen production."
Sieber J.R., Sims D.R., Han C., Kim E., Lykidis A., Lapidus A.L., McDonnald E., Rohlin L., Culley D.E., Gunsalus R., McInerney M.J.
Environ. Microbiol. 12:2289-2301(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 2245B / Goettingen.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000448 Genomic DNA. Translation: ABI68900.1.
RefSeqYP_754271.1. NC_008346.1.

3D structure databases

ProteinModelPortalQ0AWK4.
SMRQ0AWK4. Positions 1-759.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING335541.Swol_1599.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABI68900; ABI68900; Swol_1599.
GeneID4283694.
KEGGswo:Swol_1599.
PATRIC23858145. VBISynWol51738_1718.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycSWOL335541:GHL1-1645-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SYNWW
AccessionPrimary (citable) accession number: Q0AWK4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 17, 2006
Last modified: May 14, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries