Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q0AVB9

- Q0AVB9_SYNWW

UniProt

Q0AVB9 - Q0AVB9_SYNWW

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Syntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen)
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (17 Oct 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei11 – 111ATP; via amide nitrogenUniRule annotation
    Metal bindingi103 – 1031Magnesium; catalyticUniRule annotation
    Active sitei127 – 1271Proton acceptorUniRule annotation
    Binding sitei153 – 1531Allosteric activator ADPUniRule annotation
    Binding sitei161 – 1611Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei210 – 2101Allosteric activator ADPUniRule annotation
    Binding sitei221 – 2211SubstrateUniRule annotation
    Binding sitei242 – 2421Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi72 – 732ATPUniRule annotation
    Nucleotide bindingi102 – 1054ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSWOL335541:GHL1-2097-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:Swol_2041Imported
    OrganismiSyntrophomonas wolfei subsp. wolfei (strain DSM 2245B / Goettingen)Imported
    Taxonomic identifieri335541 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesSyntrophomonadaceaeSyntrophomonas
    ProteomesiUP000001968: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Protein-protein interaction databases

    STRINGi335541.Swol_2041.

    Structurei

    3D structure databases

    ProteinModelPortaliQ0AVB9.
    SMRiQ0AVB9. Positions 1-318.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni21 – 255Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni125 – 1273Substrate bindingUniRule annotation
    Regioni168 – 1703Substrate bindingUniRule annotation
    Regioni184 – 1863Allosteric activator ADP bindingUniRule annotation
    Regioni212 – 2143Allosteric activator ADP bindingUniRule annotation
    Regioni248 – 2514Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248869.
    KOiK00850.
    OMAiGFGGRCV.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q0AVB9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQKVGILTSG GDASGMNAAI RAVVRTALYH QMEAYGIHLG FEGLMAGNFD    50
    PMSRGSVADI IHRGGTILQT SRSQIFMTGN GREKARDELQ KRGIQNLVII 100
    GGNGSLRGGY ELGKLGINVI GIPATIDNDI VYTRSIGFDT AVNTALEAIN 150
    RIRDTATSHG RIFIIEVMGR HCGEIALAAG VAGGAESILI PEIETDLDEV 200
    TRKIKQGTQR GKLHSIIILA EGVYPVMELA QEIEKRTGKD TRVSILGHTQ 250
    RGGTPTAVDR IMASRMGMAA VDFIVEGKRN IMVAEEGDRI LPVPLQEVIR 300
    GTRTPELAMF EIARILSI 318
    Length:318
    Mass (Da):34,255
    Last modified:October 17, 2006 - v1
    Checksum:iEE85DD34FFE37912
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000448 Genomic DNA. Translation: ABI69335.1.
    RefSeqiYP_754706.1. NC_008346.1.

    Genome annotation databases

    EnsemblBacteriaiABI69335; ABI69335; Swol_2041.
    GeneIDi4283264.
    KEGGiswo:Swol_2041.
    PATRICi23859158. VBISynWol51738_2214.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000448 Genomic DNA. Translation: ABI69335.1 .
    RefSeqi YP_754706.1. NC_008346.1.

    3D structure databases

    ProteinModelPortali Q0AVB9.
    SMRi Q0AVB9. Positions 1-318.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 335541.Swol_2041.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABI69335 ; ABI69335 ; Swol_2041 .
    GeneIDi 4283264.
    KEGGi swo:Swol_2041.
    PATRICi 23859158. VBISynWol51738_2214.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248869.
    KOi K00850.
    OMAi GFGGRCV.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci SWOL335541:GHL1-2097-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of Syntrophomonas wolfei: new insights into syntrophic metabolism and biohydrogen production."
      Sieber J.R., Sims D.R., Han C., Kim E., Lykidis A., Lapidus A.L., McDonnald E., Rohlin L., Culley D.E., Gunsalus R., McInerney M.J.
      Environ. Microbiol. 12:2289-2301(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 2245B / GoettingenImported.

    Entry informationi

    Entry nameiQ0AVB9_SYNWW
    AccessioniPrimary (citable) accession number: Q0AVB9
    Entry historyi
    Integrated into UniProtKB/TrEMBL: October 17, 2006
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3